Assessing Flowering Time Under Different Photoperiods.
Arabidopsis thaliana
Days until bolting
Floral induction
Flowering time
Photoperiod pathway
Total leaf number
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
25
4
2022
pubmed:
26
4
2022
medline:
28
4
2022
Statut:
ppublish
Résumé
Flowering time is one of the most important developmental transitions in plants, especially in annuals such as Arabidopsis thaliana. However, flowering is also a critical agronomic trait, as it impacts the level of vegetative biomass produced (e.g., leaves) or the amount of seed (grain) generated. Therefore, uncovering flowering phenotypes would help understand the impact of any regulatory network on the overall plant life cycle, since flowering integrates multiple cues, both environmental (e.g., photoperiod, temperature) and internal (e.g., induction/repression of specific genes, phytohormone accumulation, plant age). Although the photoperiod flowering pathway has been extensively studied, and its gene circuitry characterized in great detail, specific flowering time protocols are mostly accessible to specialized laboratories in this field. In this report, we address this knowledge gap by generating a reproducible, non-expensive, and step-by-step protocol to assess flowering time under different photoperiods. We provide a comprehensive description and highlight the major pitfalls in the process. Moreover, this protocol could be expanded to include temperature changes and thus contribute to assess the impact of both environmental conditions in the plant's decision to flower.
Identifiants
pubmed: 35467202
doi: 10.1007/978-1-0716-2297-1_7
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
101-115Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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