Highly conserved protein Rv1211 in Mycobacterium tuberculosis is a natively unfolded protein that binds to a calmodulin antagonist, trifluoperazine.

Calcium / metabolism Calmodulin / metabolism EF Hand Motifs Intrinsically Disordered Proteins / metabolism Mycobacterium tuberculosis / metabolism Trifluoperazine / chemistry
Calmodulin Rv1211 Trifluoperazine

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
25 06 2022
Historique:
received: 28 03 2022
accepted: 10 04 2022
pubmed: 26 4 2022
medline: 18 5 2022
entrez: 25 4 2022
Statut: ppublish

Résumé

Rv1211 is a conserved hypothetical protein in Mycobacterium tuberculosis and is required for the growth and pathogenesis of the bacteria. The protein has been suggested as a calmodulin-like calcium-binding protein with an EF-hand motif and as a target of trifluoperazine, a calmodulin antagonist in eukaryotes that inhibits mycobacterial growth. Here, we expressed the recombinant protein of Rv1211 and performed structural and biochemical studies of Rv1211 and its interaction with Ca

Identifiants

DOI: 10.1016/j.bbrc.2022.04.045 PMID: 35468422
pubmed: 35468422
pii: S0006-291X(22)00568-X
doi: 10.1016/j.bbrc.2022.04.045
pii:
doi:

Substances chimiques

Calmodulin 0
Intrinsically Disordered Proteins 0
Trifluoperazine 214IZI85K3
Calcium SY7Q814VUP

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

182-187

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no conflicts of interest.

Auteurs

Munki Choo (M)

Natural Product Research Institute, College of Pharmacy, Seoul National University, Gwanak-Ro 1, Gwanak-gu, Seoul, 08826, Republic of Korea.

Sehyun Oh (S)

Natural Product Research Institute, College of Pharmacy, Seoul National University, Gwanak-Ro 1, Gwanak-gu, Seoul, 08826, Republic of Korea.

Sihyang Jo (S)

Natural Product Research Institute, College of Pharmacy, Seoul National University, Gwanak-Ro 1, Gwanak-gu, Seoul, 08826, Republic of Korea.

Xing Jin (X)

Natural Product Research Institute, College of Pharmacy, Seoul National University, Gwanak-Ro 1, Gwanak-gu, Seoul, 08826, Republic of Korea.

Yonghyun Song (Y)

Department of Biochemistry, Inha University Hospital, College of Medicine, Inha University, Incheon, 22212, Republic of Korea.

He Wen (H)

Department of Biochemistry and Molecular Biology, School of Medicine, Shenzhen University, Shenzhen, 518060, China.

Sunghyouk Park (S)

Natural Product Research Institute, College of Pharmacy, Seoul National University, Gwanak-Ro 1, Gwanak-gu, Seoul, 08826, Republic of Korea. Electronic address: psh@snu.ac.kr.

Sunmi Kang (S)

Natural Product Research Institute, College of Pharmacy, Seoul National University, Gwanak-Ro 1, Gwanak-gu, Seoul, 08826, Republic of Korea. Electronic address: ta-35@hanmail.net.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Facteurs de la coagulation sanguine › Calcium › Highly conserved protein Rv1211 in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines intracellulaires sensibles au calcium › Calmoduline › Highly conserved protein Rv1211 in...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Motifs à hélice-boucle-hélice › Motifs EF Hands › Highly conserved protein Rv1211 in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines intrinsèquement désordonnées › Highly conserved protein Rv1211 in...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis › Highly conserved protein Rv1211 in...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérocycliques 3 noyaux › Phénothiazines › Trifluopérazine › Highly conserved protein Rv1211 in...