[Development of an LB cloning system and its application in expression of fusion genes in

Base Sequence Cloning, Molecular Fermentation Plasmids / genetics Sphingomonas / genetics
LB cloning system Sphingomonas sp. WG gene fusion glycosyltransferase sphingan

Journal

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
ISSN: 1872-2075
Titre abrégé: Sheng Wu Gong Cheng Xue Bao
Pays: China
ID NLM: 9426463

Informations de publication

Date de publication:
25 Apr 2022
Historique:
entrez: 26 4 2022
pubmed: 27 4 2022
medline: 28 4 2022
Statut: ppublish

Résumé

In order to overcome the challenges of insufficient restriction enzyme sites, and construct a fusion-expression vector with flexible fusion direction, we designed an LB cloning system based on the type IIS and type IIT restriction enzymes

Identifiants

DOI: 10.13345/j.cjb.210440 PMID: 35470628
pubmed: 35470628
doi: 10.13345/j.cjb.210440
doi:

Types de publication

Journal Article

Langues

chi

Sous-ensembles de citation

IM

Pagination

1576-1588

Auteurs

Han Xue (H)

State Key Laboratory of Heavy Oil Processing, Department of Biological and Energy Chemical eEgineering, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong, China.

Hui Li (H)

State Key Laboratory of Heavy Oil Processing, Department of Biological and Energy Chemical eEgineering, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong, China.

Mengqi Chen (M)

State Key Laboratory of Heavy Oil Processing, Department of Biological and Energy Chemical eEgineering, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong, China.

Zaimei Zhang (Z)

State Key Laboratory of Heavy Oil Processing, Department of Biological and Energy Chemical eEgineering, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong, China.

Zhongrui Guo (Z)

State Key Laboratory of Heavy Oil Processing, Department of Biological and Energy Chemical eEgineering, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong, China.

Hu Zhu (H)

Engineering Research Center of Industrial Biocatalysis, College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, Fujian, China.

Jiqian Wang (J)

State Key Laboratory of Heavy Oil Processing, Department of Biological and Energy Chemical eEgineering, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong, China.

Yawei Sun (Y)

State Key Laboratory of Heavy Oil Processing, Department of Biological and Energy Chemical eEgineering, College of Chemistry and Chemical Engineering, China University of Petroleum (East China), Qingdao 266580, Shandong, China.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › [Development of an LB cloning system and its...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › [Development of an LB cloning system and its...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › [Development of an LB cloning system and its...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › [Development of an LB cloning system and its...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Sphingomonadaceae › Sphingomonas › [Development of an LB cloning system and its...