Dimerization of the pulmonary surfactant protein C in a membrane environment.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
05
01
2022
accepted:
01
04
2022
entrez:
27
4
2022
pubmed:
28
4
2022
medline:
30
4
2022
Statut:
epublish
Résumé
Surfactant protein C (SP-C) has several functions in pulmonary surfactant. These include the transfer of lipids between different membrane structures, a role in surfactant recycling and homeostasis, and involvement in modulation of the innate defense system. Despite these important functions, the structures of functional SP-C complexes have remained unclear. SP-C is known to exist as a primarily α-helical structure with an apparently unstructured N-terminal region, yet there is recent evidence that the functions of SP-C could be associated with the formation of SP-C dimers and higher oligomers. In this work, we used molecular dynamics simulations, two-dimensional umbrella sampling, and well-tempered metadynamics to study the details of SP-C dimerization. The results suggest that SP-C dimerizes in pulmonary surfactant membranes, forming dimers of different topologies. The simulations identified a dimerization motif region V21xxxVxxxGxxxM33 that is much larger than the putative A30xxxG34 motif that is commonly assumed to control the dimerization of some α-helical transmembrane domains. The results provide a stronger basis for elucidating how SP-C functions in concert with other surfactant proteins.
Identifiants
pubmed: 35476695
doi: 10.1371/journal.pone.0267155
pii: PONE-D-21-41017
pmc: PMC9045638
doi:
Substances chimiques
Pulmonary Surfactant-Associated Protein C
0
Pulmonary Surfactant-Associated Proteins
0
Pulmonary Surfactants
0
Surface-Active Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0267155Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Biophys J. 1997 Jun;72(6):2638-50
pubmed: 9168039
Eur Respir Rev. 2012 Jun 1;21(124):152-60
pubmed: 22654088
J Biol Chem. 2018 Jun 15;293(24):9399-9411
pubmed: 29700110
J Chem Theory Comput. 2014 Jun 10;10(6):2577-82
pubmed: 26580777
J Comput Chem. 2008 Aug;29(11):1859-65
pubmed: 18351591
Biophys J. 2015 Oct 20;109(8):1528-32
pubmed: 26488642
PLoS Comput Biol. 2017 Oct 30;13(10):e1005831
pubmed: 29084218
Chem Phys Lipids. 2006 Jun;141(1-2):105-18
pubmed: 16600200
Eur J Biochem. 1997 Mar 15;244(3):675-93
pubmed: 9108235
Biochemistry. 1995 Mar 28;34(12):3964-71
pubmed: 7696261
J Comput Chem. 2005 Dec;26(16):1701-18
pubmed: 16211538
Eur J Biochem. 2004 Jun;271(11):2086-92
pubmed: 15153098
Biochim Biophys Acta. 1998 Nov 19;1408(2-3):79-89
pubmed: 9813251
Chest. 2002 Mar;121(3 Suppl):20S-21S
pubmed: 11893657
Trends Biochem Sci. 2002 Jul;27(7):329-32
pubmed: 12114016
J Chem Theory Comput. 2008 May;4(5):819-34
pubmed: 26621095
Biochim Biophys Acta. 2013 Aug;1828(8):1829-33
pubmed: 23562404
J Mol Graph. 1996 Feb;14(1):33-8, 27-8
pubmed: 8744570
Am J Respir Crit Care Med. 2008 Oct 15;178(8):838-46
pubmed: 18635891
Biochim Biophys Acta. 2008 Jul-Aug;1778(7-8):1676-95
pubmed: 18515069
Biochemistry. 1994 May 17;33(19):6015-23
pubmed: 8180229
Biophys J. 2006 Apr 15;90(8):2698-705
pubmed: 16443648
Biophys J. 1996 Jul;71(1):246-56
pubmed: 8804608
Phys Rev A Gen Phys. 1985 Mar;31(3):1695-1697
pubmed: 9895674
J Comput Chem. 2009 Oct;30(13):2157-64
pubmed: 19229944
Biophys J. 2016 Oct 18;111(8):1703-1713
pubmed: 27760357
Ann Anat. 2017 Jan;209:78-92
pubmed: 27773772
Biochim Biophys Acta. 2014 Jun;1838(6):1568-85
pubmed: 24525076
Proc Natl Acad Sci U S A. 2001 May 22;98(11):6366-71
pubmed: 11344267
Swiss Med Wkly. 2003 May 17;133(19-20):275-82
pubmed: 12844270
Biophys J. 2008 Feb 15;94(4):1542-3; discussion 1544
pubmed: 18055534
Biochim Biophys Acta Biomembr. 2021 Jun 1;1863(6):183572
pubmed: 33548215
FEBS Lett. 2005 Mar 14;579(7):1633-8
pubmed: 15757653
J Pediatr. 2001 Jul;139(1):85-92
pubmed: 11445799
N Engl J Med. 2001 Feb 22;344(8):573-9
pubmed: 11207353
J Chem Inf Model. 2015 Oct 26;55(10):2206-17
pubmed: 26397014
J Chem Phys. 2007 Jan 7;126(1):014101
pubmed: 17212484
Eur Respir J. 2004 Jul;24(1):30-9
pubmed: 15293602
J Chem Theory Comput. 2013 Jan 8;9(1):687-97
pubmed: 26589065
J Phys Chem B. 2007 Jul 12;111(27):7812-24
pubmed: 17569554