Engineering Binders with Exceptional Selectivity.

Antigens Peptide Library Protein Engineering Saccharomyces cerevisiae / genetics
Cell sorting Protein engineering Specificity Synthetic binding protein Yeast display

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 28 4 2022
pubmed: 29 4 2022
medline: 3 5 2022
Statut: ppublish

Résumé

Molecular display technologies have enabled the generation of synthetic binders with high affinities against a variety of antigens. However, engineering binders with high selectivity is still a challenging task. Here, we illustrate points to consider in developing highly selective binders against antigens of interest. We describe a systematic strategy for sorting selective binders using the yeast display technology. Using the approach described, our group has overcome molecular recognition challenges and developed a series of synthetic binders with exceptional selectivity against diverse antigens.

Identifiants

DOI: 10.1007/978-1-0716-2285-8_8 PMID: 35482189
pubmed: 35482189
doi: 10.1007/978-1-0716-2285-8_8
doi:

Substances chimiques

Antigens 0
Peptide Library 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

143-154

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Kai Wen Teng (KW)

Perlmutter Cancer Center, New York University Langone Medical Center, New York, NY, USA.
Discovery Biologics, Merck & Co., Inc., Boston, MA, USA.

Akiko Koide (A)

Perlmutter Cancer Center, New York University Langone Medical Center, New York, NY, USA.
Department of Medicine, New York University School of Medicine, New York, NY, USA.

Shohei Koide (S)

Perlmutter Cancer Center, New York University Langone Medical Center, New York, NY, USA. Shohei.Koide@nyulangone.org.
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA. Shohei.Koide@nyulangone.org.

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Antigènes › Engineering Binders with Exceptional Selectivity.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Banque de gènes › Banque de peptides › Engineering Binders with Exceptional Selectivity.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Ingénierie des protéines › Engineering Binders with Exceptional Selectivity.
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Engineering Binders with Exceptional Selectivity.