Epigenetic mechanisms affect the curled leaf phenotype in the hypomethylated ddc mutant of Arabidopsis thaliana.

Arabidopsis / genetics Arabidopsis Proteins / genetics Epigenesis, Genetic Gene Expression Regulation, Plant Indoleacetic Acids / metabolism Phenotype Transcription Factors / genetics
Arabidopsis thaliana ChIP-qPCR Curled leaf Epigenomics WGBS ddc mutant

Journal

Plant science : an international journal of experimental plant biology
ISSN: 1873-2259
Titre abrégé: Plant Sci
Pays: Ireland
ID NLM: 9882015

Informations de publication

Date de publication:
Jun 2022
Historique:
received: 21 09 2021
revised: 02 02 2022
accepted: 12 03 2022
entrez: 29 4 2022
pubmed: 30 4 2022
medline: 4 5 2022
Statut: ppublish

Résumé

The ddc mutant of Arabidopsis thaliana is characterized by pleiotropic phenotypic alterations including a curl-shaped leaf, previously explained by disturbed auxin metabolism and transport. The present study was aimed at further explore the molecular bases underlying the abnormal phenotype of the ddc leaf. We demonstrated that genes specifically related to leaf fate commitment and morphogenesis were misexpressed on developing ddc leaves, such as upregulation of CURLY LEAF (CLF) and downregulation of ASYMMETRIC LEAVES2 (AS2), KNOTTED-like gene from A. thaliana (KNAT6), TEOSINTE-LIKE1 CYCLOIDEA and PROLIFERATING CELL FACTOR 2 (TCP2) and others. The CLF gene, encoding a component of Polycomb repressive complex 2 (PRC2) which adds trimethylation marks at Lys27 of histone H3, was overexpressed in the ddc mutant and concomitantly was correlated with DNA methylation-dependent repression of its negative regulator UCL1. KNAT6, encoding a class 1 KNOX homeotic gene, had increased H3K27me3 trimethylation levels, suggesting it is a target gene of the CLF containing PRC2 complex in the ddc mutant. We postulate that different epigenetic mechanisms modulate expression of genes related to auxin pathways as well as gene targets of Polycomb repressive action, during leaf morphogenesis.

Identifiants

DOI: 10.1016/j.plantsci.2022.111254 PMID: 35487663
pubmed: 35487663
pii: S0168-9452(22)00078-4
doi: 10.1016/j.plantsci.2022.111254
pii:
doi:

Substances chimiques

Arabidopsis Proteins 0
Indoleacetic Acids 0
KNAT6 protein, Arabidopsis 0
Transcription Factors 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

111254

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Ivano Forgione (I)

Department of Biology, Ecology and Earth Science, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy; Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Center Plant Systems Biology, VIB, 9052 Ghent, Belgium. Electronic address: ivanoforgione@gmail.com.

Antonella Muto (A)

Department of Biology, Ecology and Earth Science, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy. Electronic address: antonella.muto@unical.it.

Magdalena Woloszynska (M)

Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Center Plant Systems Biology, VIB, 9052 Ghent, Belgium; Department of Genetics, Faculty of Biology and Animal Sciences, Wroclaw University of Environmental and Life Sciences, ul. Kozuchowska 7, 51-631 Wroclaw, Poland. Electronic address: magdalena.woloszynska@upwr.edu.pl.

Adriana Ada Chiappetta (AA)

Department of Biology, Ecology and Earth Science, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy. Electronic address: adriana.chiappetta@unical.it.

Michele Ferrari (M)

Department of Biology, Ecology and Earth Science, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy. Electronic address: michele.ferrari@unical.it.

Mieke Van Lijsebettens (M)

Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium; Center Plant Systems Biology, VIB, 9052 Ghent, Belgium. Electronic address: mieke.vanlijsebettens@psb.vib-ugent.be.

Maria Beatrice Bitonti (MB)

Department of Biology, Ecology and Earth Science, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy. Electronic address: maria_beatrice.bitonti@unical.it.

Leonardo Bruno (L)

Department of Biology, Ecology and Earth Science, University of Calabria, Ponte P. Bucci, Arcavacata di Rende, 87036 Cosenza, Italy. Electronic address: leonardo.bruno@unical.it.

Articles similaires

Multiple NADPH-cytochrome P450 reductases from Lycoris radiata involved in Amaryllidaceae alkaloids biosynthesis.

Yuqing Wu, Yifeng Zhang, Haitong Fan et al.
1.00
Amaryllidaceae Alkaloids Lycoris NADPH-Ferrihemoprotein Reductase Gene Expression Regulation, Plant Plant Proteins

Systematical characterization of Rab7 gene family in Gossypium and potential functions of GhRab7B3-A gene in drought tolerance.

Mengyuan Yan, Zhiwei Dong, Tian Pan et al.
1.00
Drought Resistance Gene Expression Profiling Gene Expression Regulation, Plant Gossypium Multigene Family

A cytoplasmic osmosensing mechanism mediated by molecular crowding-sensitive DCP5.

Zhenyu Wang, Qiuhua Yang, Dan Zhang et al.
1.00
Arabidopsis Arabidopsis Proteins Osmotic Pressure Cytoplasm RNA, Messenger

Lipid accumulation in adipose tissue-resident iNKT cells contributes to an inflammatory phenotype.

Imogen Morris, Frank Vrieling, Annemieke Bouwman et al.
1.00
Animals Natural Killer T-Cells Mice Adipose Tissue Lipid Metabolism

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Epigenetic mechanisms affect the curled leaf...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Epigenetic mechanisms affect the curled leaf...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Epigenetic mechanisms affect the curled leaf...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Epigenetic mechanisms affect the curled leaf...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Acides indolacétiques › Epigenetic mechanisms affect the curled leaf...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Epigenetic mechanisms affect the curled leaf...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Epigenetic mechanisms affect the curled leaf...