Genome-wide association study identifies loci and candidate genes for grain micronutrients and quality traits in wheat (Triticum aestivum L.).


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
29 04 2022
Historique:
received: 31 12 2021
accepted: 08 04 2022
entrez: 29 4 2022
pubmed: 30 4 2022
medline: 4 5 2022
Statut: epublish

Résumé

Malnutrition due to micronutrients and protein deficiency is recognized among the major global health issues. Genetic biofortification of wheat is a cost-effective and sustainable strategy to mitigate the global micronutrient and protein malnutrition. Genomic regions governing grain zinc concentration (GZnC), grain iron concentration (GFeC), grain protein content (GPC), test weight (TW), and thousand kernel weight (TKW) were investigated in a set of 184 diverse bread wheat genotypes through genome-wide association study (GWAS). The GWAS panel was genotyped using Breeders' 35 K Axiom Array and phenotyped in three different environments during 2019-2020. A total of 55 marker-trait associations (MTAs) were identified representing all three sub-genomes of wheat. The highest number of MTAs were identified for GPC (23), followed by TKW (15), TW (11), GFeC (4), and GZnC (2). Further, a stable SNP was identified for TKW, and also pleiotropic regions were identified for GPC and TKW. In silico analysis revealed important putative candidate genes underlying the identified genomic regions such as F-box-like domain superfamily, Zinc finger CCCH-type proteins, Serine-threonine/tyrosine-protein kinase, Histone deacetylase domain superfamily, and SANT/Myb domain superfamily proteins, etc. The identified novel MTAs will be validated to estimate their effects in different genetic backgrounds for subsequent use in marker-assisted selection.

Identifiants

pubmed: 35487909
doi: 10.1038/s41598-022-10618-w
pii: 10.1038/s41598-022-10618-w
pmc: PMC9054743
doi:

Substances chimiques

Micronutrients 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7037

Informations de copyright

© 2022. The Author(s).

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Auteurs

Nagenahalli Dharmegowda Rathan (ND)

Indian Agricultural Research Institute, New Delhi, India.

Hari Krishna (H)

Indian Agricultural Research Institute, New Delhi, India.

Ranjith Kumar Ellur (RK)

Indian Agricultural Research Institute, New Delhi, India.

Deepmala Sehgal (D)

International Maize and Wheat Improvement Center, Texcoco, Mexico.

Velu Govindan (V)

International Maize and Wheat Improvement Center, Texcoco, Mexico. velu@cgiar.org.

Arvind Kumar Ahlawat (AK)

Indian Agricultural Research Institute, New Delhi, India.

Gopalareddy Krishnappa (G)

Indian Institute of Wheat and Barley Research, Karnal, India.

Jai Prakash Jaiswal (JP)

Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, India.

Jang Bahadur Singh (JB)

Indian Agricultural Research Institute, Regional Station, Indore, India.

Saiprasad Sv (S)

Indian Agricultural Research Institute, Regional Station, Indore, India.

Divya Ambati (D)

Indian Agricultural Research Institute, Regional Station, Indore, India.

Sumit Kumar Singh (SK)

Indian Agricultural Research Institute, New Delhi, India.

Kriti Bajpai (K)

Indian Agricultural Research Institute, New Delhi, India.

Anju Mahendru-Singh (A)

Indian Agricultural Research Institute, New Delhi, India. anju_mahendru@yahoo.co.in.

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