Cellular point-of-care diagnostics using an inexpensive layer-stack microfluidic device.
Journal
Lab on a chip
ISSN: 1473-0189
Titre abrégé: Lab Chip
Pays: England
ID NLM: 101128948
Informations de publication
Date de publication:
31 05 2022
31 05 2022
Historique:
pubmed:
7
5
2022
medline:
3
6
2022
entrez:
6
5
2022
Statut:
epublish
Résumé
Cellular analyses are increasingly used to diagnose diseases at point-of-care and global healthcare settings. Some analyses are simple as they rely on chromogenic stains (blood counts, malaria) but others often require higher multiplexing to define and quantitate cell populations (cancer diagnosis, immunoprofiling). Simplifying the latter with inexpensive solutions represents a current bottleneck in designing start-end pipelines. Based on the hypothesis that novel film adhesives could be used to create inexpensive disposable devices, we tested a number of different designs and materials, to rapidly perform 12-15 channel single-cell imaging. Using an optimized passive pumping layer-stack microfluidic (PLASMIC) device (<1 $ in supplies) we show that rapid, inexpensive cellular analysis is feasible.
Identifiants
pubmed: 35514273
doi: 10.1039/d2lc00162d
pmc: PMC9214713
mid: NIHMS1817135
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2145-2154Subventions
Organisme : NCI NIH HHS
ID : R01 CA257623
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA236561
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA079443
Pays : United States
Références
Cell. 2020 Dec 10;183(6):1665-1681.e18
pubmed: 33188776
Lab Chip. 2008 Feb;8(2):198-220
pubmed: 18231657
Cytometry B Clin Cytom. 2015 Jan;88(1):64-8
pubmed: 25196066
Nat Commun. 2015 Sep 24;6:8390
pubmed: 26399630
RSC Adv. 2020 Mar 23;10(20):11652-11680
pubmed: 35496619
Cell. 2018 Aug 9;174(4):968-981.e15
pubmed: 30078711
Biomed Microdevices. 2011 Feb;13(1):203-13
pubmed: 20978853
Sci Transl Med. 2020 Aug 5;12(555):
pubmed: 32759277
Adv Chronic Kidney Dis. 2013 Nov;20(6):508-15
pubmed: 24206603
Clin Cancer Res. 2021 Sep 1;27(17):4781-4793
pubmed: 34233961
JCI Insight. 2018 Nov 2;3(21):
pubmed: 30385729
Adv Mater Technol. 2019 Nov;4(11):
pubmed: 32395607
Nat Commun. 2019 May 15;10(1):2163
pubmed: 31092822
BMJ. 2004 Jul 31;329(7460):244-5
pubmed: 15284126
Immunity. 2018 Dec 18;49(6):1148-1161.e7
pubmed: 30552023
Proc Natl Acad Sci U S A. 2014 May 13;111(19):7048-53
pubmed: 24782542
JCO Precis Oncol. 2017 Nov;1:1-9
pubmed: 35172508
Theranostics. 2019 Nov 26;9(26):8426-8436
pubmed: 31879528
Int J Mol Sci. 2018 Oct 12;19(10):
pubmed: 30322072
Micromachines (Basel). 2018 Sep 12;9(9):
pubmed: 30424394
Sci Adv. 2020 Nov 20;6(47):
pubmed: 33219024
Angew Chem Weinheim Bergstr Ger. 2020 Apr 20;132(17):6906-6913
pubmed: 34366494
J Cereb Blood Flow Metab. 2019 Mar;39(3):395-410
pubmed: 30565961