Heterologous Expression, Purification, and Characterization of Type II Polyketide Synthase Acyl Carrier Proteins.
Acyl carrier protein
Heterologous expression
Polyaromatic polyketide
Polyketide synthase
Type II polyketide
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
6
5
2022
pubmed:
7
5
2022
medline:
11
5
2022
Statut:
ppublish
Résumé
The enzymes that comprise type II polyketide synthases (PKSs) are powerful biocatalysts that, once well-understood and strategically applied, could enable cost-effective and sustainable access to a range of pharmaceutically relevant molecules. Progress toward this goal hinges on gaining ample access to materials for in vitro characterizations and structural analysis of the components of these synthases. A central component of PKSs is the acyl carrier protein (ACP), which serves as a hub during the biosynthesis of type II polyketides. Herein, we share methods for accessing type II PKS ACPs via heterologous expression in E. coli . We also share how the installation of reactive and site-specific spectroscopic probes can be leveraged to study the conformational dynamics and interactions of type II PKS ACPs.
Identifiants
pubmed: 35524054
doi: 10.1007/978-1-0716-2273-5_13
pmc: PMC9373356
mid: NIHMS1826209
doi:
Substances chimiques
Acyl Carrier Protein
0
Polyketide Synthases
79956-01-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
239-267Subventions
Organisme : NIGMS NIH HHS
ID : R15 GM120704
Pays : United States
Informations de copyright
© 2022. The Author(s).
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