Whole-genome sequencing and mutational analysis of human cord-blood derived stem and progenitor cells.
Bioinformatics
Cancer
Cell isolation
Flow Cytometry/Mass Cytometry
Genomics
Molecular Biology
Sequence analysis
Sequencing
Single Cell
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 06 2022
17 06 2022
Historique:
entrez:
16
5
2022
pubmed:
17
5
2022
medline:
18
5
2022
Statut:
epublish
Résumé
Mutational signatures have been identified in cancer genomes, providing information about the causes of cancer and treatment vulnerabilities. This protocol describes an assay to determine the genotoxic mechanisms underlying these signatures using cord-blood derived hematopoietic stem and progenitor cells (CB-HSPCs). CB-HSPCs have a low mutation background, enabling sensitive detection of mutations. First, CB-HSPCs are exposed
Identifiants
pubmed: 35573477
doi: 10.1016/j.xpro.2022.101361
pii: S2666-1667(22)00241-6
pmc: PMC9092504
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101361Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
A.R.H., A.v.L., and R.v.B. are named as inventors on a patent application filed resulting from this work.
Références
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