High-performance thin-layer chromatography in combination with an acetylcholinesterase-inhibition bioassay with pre-oxidation of organothiophosphates to determine neurotoxic effects in storm, waste, and surface water.
Acetylcholinesterase-inhibition assay (AChE-I assay)
High-performance thin-layer chromatography (HPTLC)
Organothiophosphates
Oxidation by N-bromosuccinimide
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
24
05
2021
accepted:
05
04
2022
revised:
26
02
2022
pubmed:
19
5
2022
medline:
25
5
2022
entrez:
18
5
2022
Statut:
ppublish
Résumé
Pesticides such as organothiophosphates (OTPs) are neurotoxically active and enter the aquatic environment. Bioassays, using acetylcholinesterase (AChE), a suitable substrate and reactant, can be applied for the photometric detection of AChE-inhibiton (AChE-I) effects. The oxidized forms of OTPs, so-called oxons, have higher inhibition potentials for AChE. Therefore, a higher sensitivity is achieved for application of oxidized samples to the AChE assay. In this study, the oxidation of malathion, parathion, and chlorpyrifos by n-bromosuccinimide (NBS) was investigated in an approach combining high-performance thin-layer chromatography (HPTLC) with an AChE-I assay. Two AChE application approaches, immersion and spraying, were compared regarding sensitivity, precision, and general feasibility of the OTP effect detection. The oxidation by NBS led to an activation of the OTPs and a strong increase in sensitivity similar to the oxons tested. The sensitivity and precision of the two application techniques were similar, although the spray method was slightly more sensitive to the oxidized OTPs. The 10% inhibition concentrations (IC
Identifiants
pubmed: 35583680
doi: 10.1007/s00216-022-04068-6
pii: 10.1007/s00216-022-04068-6
pmc: PMC9124651
doi:
Substances chimiques
Cholinesterase Inhibitors
0
Insecticides
0
Organothiophosphates
0
Water
059QF0KO0R
Parathion
61G466064D
Acetylcholinesterase
EC 3.1.1.7
Chlorpyrifos
JCS58I644W
Malathion
U5N7SU872W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4167-4178Informations de copyright
© 2022. The Author(s).
Références
J Toxicol Environ Health. 1994 Nov;43(3):271-89
pubmed: 7966438
Drug Metab Dispos. 2001 Sep;29(9):1201-4
pubmed: 11502728
Anal Biochem. 1987 Nov 1;166(2):399-403
pubmed: 3434781
J Chromatogr. 1968 Aug 27;36(3):309-17
pubmed: 5683240
Environ Pollut. 2010 Oct;158(10):3054-64
pubmed: 20663596
J Chromatogr A. 2020 Aug 2;1624:461239
pubmed: 32540077
Anal Chim Acta. 2020 Sep 8;1129:76-84
pubmed: 32891393
J Hazard Mater. 2006 Aug 25;136(3):553-9
pubmed: 16436313
Water Res. 2014 Sep 1;60:64-74
pubmed: 24830785
Talanta. 2016 Aug 1;155:289-304
pubmed: 27216686
Water Sci Technol. 2011;63(9):1974-82
pubmed: 21902038
Toxicol Sci. 1999 Jan;47(1):16-22
pubmed: 10048149
J Chromatogr A. 2020 Oct 11;1629:461511
pubmed: 32896687
Chemosphere. 2011 Jan;82(3):291-307
pubmed: 21055790
J Chromatogr A. 2017 Nov 17;1524:273-282
pubmed: 29031972
J Agric Food Chem. 2010 Aug 11;58(15):8748-56
pubmed: 20614938
Chemosphere. 2020 Dec;261:127706
pubmed: 32717513
Biochem Pharmacol. 1961 Jul;7:88-95
pubmed: 13726518
J Chromatogr B Analyt Technol Biomed Life Sci. 2010 May 15;878(17-18):1337-45
pubmed: 20060788
Anal Bioanal Chem. 2020 Jul;412(19):4527-4536
pubmed: 32458016
J Chromatogr A. 2017 Dec 29;1530:185-191
pubmed: 29146425
Toxicol Lett. 2000 Aug 16;116(3):217-21
pubmed: 10996483
FEMS Microbiol Rev. 2006 May;30(3):428-71
pubmed: 16594965
Anal Chem. 2004 Mar 15;76(6):1720-5
pubmed: 15018574
Drug Test Anal. 2012 Mar-Apr;4(3-4):282-91
pubmed: 21998030
J Chromatogr A. 2020 May 10;1618:460942
pubmed: 32057448
Adv Biochem Eng Biotechnol. 2017;157:187-224
pubmed: 27757476
Talanta. 2021 Feb 1;223(Pt 1):121701
pubmed: 33303153
Environ Health Perspect. 1990 Jul;87:245-54
pubmed: 2176588
Anal Bioanal Chem. 2021 Feb;413(5):1321-1335
pubmed: 33388849
Analyst. 1968 Jan;93(102):34-8
pubmed: 5636724
J Chromatogr A. 1996 Oct 25;750(1-2):403-7
pubmed: 8938396
Environ Sci Technol. 2016 Apr 19;50(8):4289-95
pubmed: 26963769
J Chromatogr A. 2011 May 13;1218(19):2775-84
pubmed: 21397236
Water Res. 2010 May;44(9):2850-62
pubmed: 20188390
J Chromatogr A. 2019 Sep 27;1602:458-466
pubmed: 31153601
Water Res. 2007 Nov;41(19):4504-14
pubmed: 17624398