The RNA-bound proteome of MRSA reveals post-transcriptional roles for helix-turn-helix DNA-binding and Rossmann-fold proteins.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 05 2022
24 05 2022
Historique:
received:
09
04
2021
accepted:
06
05
2022
entrez:
24
5
2022
pubmed:
25
5
2022
medline:
27
5
2022
Statut:
epublish
Résumé
RNA-binding proteins play key roles in controlling gene expression in many organisms, but relatively few have been identified and characterised in detail in Gram-positive bacteria. Here, we globally analyse RNA-binding proteins in methicillin-resistant Staphylococcus aureus (MRSA) using two complementary biochemical approaches. We identify hundreds of putative RNA-binding proteins, many containing unconventional RNA-binding domains such as Rossmann-fold domains. Remarkably, more than half of the proteins containing helix-turn-helix (HTH) domains, which are frequently found in prokaryotic transcription factors, bind RNA in vivo. In particular, the CcpA transcription factor, a master regulator of carbon metabolism, uses its HTH domain to bind hundreds of RNAs near intrinsic transcription terminators in vivo. We propose that CcpA, besides acting as a transcription factor, post-transcriptionally regulates the stability of many RNAs.
Identifiants
pubmed: 35610211
doi: 10.1038/s41467-022-30553-8
pii: 10.1038/s41467-022-30553-8
pmc: PMC9130240
doi:
Substances chimiques
Bacterial Proteins
0
DNA-Binding Proteins
0
Proteome
0
Transcription Factors
0
RNA
63231-63-0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2883Subventions
Organisme : Wellcome Trust
ID : 109093/Z/15/A
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 208402/Z/17/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R008205/1
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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