Medium optimization for submerged fermentative production of β-cyclodextrin glucosyltransferase by isolated novel alkalihalophilic Bacillus sp. NCIM 5799 using statistical approach.
Bacillus sp. NCIM 5799
Plackett-Burman design (PBD)
central composite design (CCD)
medium optimization
β-CGTase
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Aug 2022
Aug 2022
Historique:
revised:
14
05
2022
received:
18
03
2022
accepted:
16
05
2022
pubmed:
26
5
2022
medline:
20
8
2022
entrez:
25
5
2022
Statut:
ppublish
Résumé
β-cyclodextrin glucosyltransferase (β-CGTase) is an essential enzyme to catalyse the biotransformation of starch into β-cyclodextrins (β-CD). β-CD has widespread applications in the biomedical, pharmaceutical and food industries. The present study focused on β-CGTase production using an efficient natural microbial strain and statistical production optimization for enhanced production. The isolated organism Bacillus sp. NCIM 5799 was found to be 5 μm short bacilli under FE-SEM and alkalihalophilic in nature. The β-CGTase production was optimized using a combination of Plackett-Burman design (PBD) and Central Composite Design-Response Surface Methodology (CCD-RSM). On PBD screening Na
Substances chimiques
Peptones
0
beta-Cyclodextrins
0
Starch
9005-25-8
Glucosyltransferases
EC 2.4.1.-
betadex
JV039JZZ3A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
431-441Subventions
Organisme : National Fellowship for Higher Education of ST Students
ID : F117.1/201415/RGNF201415STMAD69908/ (SAIII/Website)
Informations de copyright
© 2022 The Society for Applied Microbiology.
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