Native ambient mass spectrometry of intact protein assemblies directly from


Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
14 Jun 2022
Historique:
pubmed: 27 5 2022
medline: 18 6 2022
entrez: 26 5 2022
Statut: epublish

Résumé

Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.

Identifiants

pubmed: 35617055
doi: 10.1039/d2cc02085h
pmc: PMC9196203
doi:

Substances chimiques

Escherichia coli Proteins 0
hdeA protein, E coli 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

6857-6860

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Auteurs

Yuying Du (Y)

School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. h.j.cooper@bham.ac.uk.

Robin C May (RC)

School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. h.j.cooper@bham.ac.uk.

Helen J Cooper (HJ)

School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK. h.j.cooper@bham.ac.uk.

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Classifications MeSH