Systematic Study of Enzymatic Degradation and Plasmid DNA Complexation of Mucus Penetrating Star-Shaped Lysine/Sarcosine Polypept(o)ides with Different Block Arrangements.
DNA polyplexes
enzymatic degradation
mucus penetration
polypept(o)ides
star polymers
Journal
Macromolecular bioscience
ISSN: 1616-5195
Titre abrégé: Macromol Biosci
Pays: Germany
ID NLM: 101135941
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
18
05
2022
received:
03
05
2022
pubmed:
1
6
2022
medline:
23
8
2022
entrez:
31
5
2022
Statut:
ppublish
Résumé
8-Arm star polypep(o)ides comprising cationic polylysine and hydrophilic polysarcosine blocks with a degree of polymerization (DP) of 30 per block are synthesized. Two different block sequences with polylysine as the inner and polysarcosine as the outer block and vice versa are obtained in addition to a statistical copolymer. Analysis of the enzymatic hydrolysis by the proteolytic enzyme trypsin demonstrates a strong dependence on structural arrangements. While polypept(o)ide disintegration is detectible after 24 h by Size Exclusion Chromatography (SEC), significant hydrolysis of the lysine blocks is only monitored after 48 h by fluorescamine labeling of the produced lysine and clearly accelerated in structures with more accessible polylysine blocks. All structures are capable of complexing plasmid DNA and form gene nanomedicines at sizes around or below 200 nm as determined by Dynamic Light Scattering (DLS), Nanoparticle Tracking Analysis (NTA), and Transition Electron Microscopy (TEM). The polyplex formation is slightly enhanced for both block structures over the random copolypept(o)ide. Moreover, it is demonstrated that the polyplexes can transport through mucus. The results highlight the importance of structural control in compartmentalized polymeric gene vector candidates with hydrophilic domains for potential mucosal delivery.
Identifiants
pubmed: 35634688
doi: 10.1002/mabi.202200175
doi:
Substances chimiques
Polymers
0
Polylysine
25104-18-1
DNA
9007-49-2
Sarcosine
Z711V88R5F
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200175Informations de copyright
© 2022 The Authors. Macromolecular Bioscience published by Wiley-VCH GmbH.
Références
C. Deng, J. Wu, R. Cheng, F. Meng, H. A. Klok, Z. Zhong, Prog. Polym. Sci. 2014, 39, 330.
X. Zhou, Z. Li, Adv. Healthcare Mat. 2018, 7, 1800020.
J. Huang, A. Heise, Chem. Soc. Rev. 2013,42, 7373.
A. Rasines Mazo, S. Allison-Logan, F. Karimi, N. J.-A.n Chan, W. Qiu, W. Duan, N. M. O'brien-Simpson, G. G. Qiao, Chem. Soc. Rev. 2020,49, 4737.
M. Byrne, R. Murphy, A. Kapetanakis, J. Ramsey, S. A. Cryan, A. Heise, Macromol. Rapid Commun. 2015, 36, 1862.
P. Zhang, M. Li, C. Xiao, X. Chen, Chem. Commun. 2021,57, 9489.
X.u Wang, Z. Song, S. Wei, G. Ji, X. Zheng, Z. Fu, J. Cheng, Biomaterials 2021, 275, 120913.
Y. Liu, L. Yin, Adv. Drug Delivery Rev. 2021, 171, 139.
T. Melnyk, S.ž Đorđević, I. Conejos-Sánchez, M. J. Vicent, Adv. Drug Delivery Rev. 2020, 160, 136.
E. Georgilis, M. Abdelghani, J. Pille, E. Aydinlioglu, J. C. M. Van Hest, S. Lecommandoux, E. Garanger, Int. J. Pharmaceutics 2020, 586, 119537.
V. Ahmadi, F. Zabihi, F. Rancan, A. A. Staszak, C. Nie, M. Dimde, K. Achazi, A. Wiehe, A. Vogt, R. Haag, ACS Appl. Nano Mater 2021, 4, 6709.
M. Byrne, D. Victory, A. Hibbitts, M. Lanigan, A. Heise, S. A. Cryan, Biomater. Sci. 2013, 1, 1223.
D. P. Walsh, R. D. Murphy, A. Panarella, R. M. Raftery, B. Cavanagh, J. C. Simpson, F. J. O'brien, A. Heise, S. A. Cryan, Mol. Pharmaceutics 2018, 15, 1878.
D. P. Walsh, R. M. Raftery, G. Chen, A. Heise, F. J. O'brien, S. A. Cryan, J. Tissue Eng. Regener. Med. 2019, 13, 1843.
J. O'dwyer, R. Murphy, E. B. Dolan, L. Kovarova, M. Pravda, V. Velebny, A. Heise, G. P. Duffy, S. A. Cryan, Drug Deliv. Transl. Res. 2020, 10, 440.
D. P. Walsh, R. M. Raftery, R. Murphy, G. Chen, A. Heise, F. J. O'brien, S. A. Cryan, Biomater. Sci. 2021, 9, 4984.
M. J. Alonso, A. Sánchez, J. Pharm. Pharmacol. 2003, 55, 1451.
B. C. Tang, J. Fu, D. N. Watkins, J. Hanes, Biomaterials 2010, 31, 339.
N. Durcan, C. Murphy, S. A. Cryan, Mol. Pharmaceutics 2008, 5, 559.
M. Ghadiri, P. Young, D. Traini, Pharmaceutics 2019, 11, 113.
L. Gomes Dos Reis, M. Svolos, B. Hartwig, N. Windhab, P. M. Young, D. Traini, Expert Opin Drug Deliv 2017, 14, 319.
R. Heida, W. L.j Hinrichs, H. W. Frijlink, Expert Rev. Vaccines 2021, https://doi.org/10.1080/14760584.2021.1903878
G. A. Duncan, J. Jung, J. Hanes, J. S. Suk, Mol. Ther. 2016, 24, 2043.
S. K. Lai, Y. Y. Wang, J. Hanes, Adv. Drug Delivery Rev 2009, 61, 158.
L. N. Thwala, D. P. Delgado, K. Leone, I. Marigo, F. Benetti, M. Chenlo, C. V. Alvarez, S. Tovar, C. Dieguez, N. S. Csaba, M. J. Alonso, J. Controlled Release 2018, 291, 157.
Y. Y. Wang, S. K. Lai, J. . S. Suk, A. Pace, R. Cone, J. Hanes, Angew. Chem., Int. Ed. 2008, 47, 9726.
Q. Xu, N. J. Boylan, J. S. Suk, Y. Y. Wang, E. A. Nance, J. C. Yang, P. J. Mcdonnell, R. A. Cone, E. J. Duh, J. Hanes, J. Controlled Release 2013, 167, 76.
D. Skoulas, V. Stuettgen, R. Gaul, S. A. Cryan, D. J. Brayden, A. Heise, Biomacromolecules 2020, 21, 2455.
B. A. Chan, S. Xuan, A. Li, J. M. Simpson, G. L. Sternhagen, T. Yu, O. A. Darvish, N. Jiang, D. Zhang, Biopolymers 2018, 109, e23070.
N. Gangloff, J. Ulbricht, T. Lorson, H. Schlaad, R. Luxenhofer, Chem. Rev. 2016, 116, 1753.
A. Birke, J. Ling, M. Barz, Prog. Polym. Sci. 2018, 81, 163.
H. Yu, N. Ingram, J. V. Rowley, S. Parkinson, D. C. Green, N. J. Warren, P. D. Thornton, J. Mater. Chem. B 2019, 7, 4217.
S. Bleher, J. Buck, C. Muhl, S. Sieber, S. Barnert, D. Witzigmann, J. Huwyler, M. Barz, R. Süss, Small 2019, 15, 1904716.
N. D. Rawlings, G. Salvesen, Handbook of Proteolytic Enzymes, Academic Press 2013.
P. Bilalis, D. Skoulas, A. Karatzas, J. Marakis, A. Stamogiannos, C. Tsimblouli, E. Sereti, E. Stratikos, K. Dimas, D. Vlassopoulos, H. Iatrou, Biomacromolecules 2018, 19, 3840.
R. R. Watson, V. Preedy (Editors), Bioactive Food as Dietary Interventions for Liver and Gastrointestinal Disease, Academic Press 2013.
B. Weber, A. Birke, K. Fischer, M. Schmidt, M. Barz, Macromolecules 2018, 51, 7, 2653.
Y. Jeong, M. K. Joo, K. H. Bahk, Y. Y. Choi, H.o-T. Kim, W.-K.i Kim, H. Jeong Lee, Y. S. Sohn, B. Jeong, J. Controlled Release 2009, 137, 25.
T. Bantan-Polak, M. Kassai, K. B. Grant, Anal. Biochem. 2001, 297, 128.
D. J. Gary, N. Puri, Y. Y. Won, J. Controlled Release 2007, 121, 64.
A. Kim, W. B. Ng, W. Bernt, N. J. Cho, Sci. Rep. 2019, 9, 2639.
D. P. Walsh, A. Heise, F. J. O'brien, S. A. Cryan, Gene Ther. 2017, 24, 681.
F. Ungaro, I. D'angelo, C. Coletta, R. d'Emmanuele Di Villa Bianca, R. Sorrentino, B. Perfetto, M. A. Tufano, A. Miro, M. I. La Rotonda, F. Quaglia, J. Controlled Release 2012, 157, 149.