Atomic Force Microscopy of DNA and DNA-Protein Interactions.

DNA / chemistry Microscopy, Atomic Force / methods Nanotechnology Proteins / metabolism
AFM Atomic force microscopy DNA DNA-protein binding Double helix Supercoiling

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 31 5 2022
pubmed: 1 6 2022
medline: 3 6 2022
Statut: ppublish

Résumé

Atomic force microscopy (AFM) is a microscopy technique that uses a sharp probe to trace a sample surface at nanometer resolution. For biological applications, one of its key advantages is its ability to visualize the substructure of single molecules and molecular complexes in an aqueous environment. Here, we describe the application of AFM to determine the secondary and tertiary structure of surface-bound DNA, and its interactions with proteins.

Identifiants

DOI: 10.1007/978-1-0716-2221-6_5 PMID: 35635696
pubmed: 35635696
doi: 10.1007/978-1-0716-2221-6_5
doi:

Substances chimiques

Proteins 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

43-62

Subventions

Organisme : Medical Research Council
ID : MR/R024871/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R024871/2
Pays : United Kingdom
Organisme : Cancer Research UK
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001119
Pays : United Kingdom

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Philip J Haynes (PJ)

London Centre for Nanotechnology, University College London, London, UK.
Molecular Sciences Research Hub, Department of Chemistry, Imperial College London, London, UK.
Department of Physics and Astronomy, University College London, London, UK.

Kavit H S Main (KHS)

London Centre for Nanotechnology, University College London, London, UK.
UCL Cancer Institute, University College London, London, UK.

Bernice Akpinar (B)

London Centre for Nanotechnology, University College London, London, UK.
Molecular Sciences Research Hub, Department of Chemistry, Imperial College London, London, UK.

Alice L B Pyne (ALB)

London Centre for Nanotechnology, University College London, London, UK. a.l.pyne@sheffield.ac.uk.
Department of Materials Science and Engineering, University of Sheffield, Sheffield, UK. a.l.pyne@sheffield.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Atomic Force Microscopy of DNA and DNA-Protein...
questionsmedicales.fr › Techniques d'investigation › Microscopie à sonde à balayage › Microscopie à force atomique › Atomic Force Microscopy of DNA and DNA-Protein...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Miniaturisation › Nanotechnologie › Atomic Force Microscopy of DNA and DNA-Protein...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Atomic Force Microscopy of DNA and DNA-Protein...