Plant Microbiome-Based Genome-Wide Association Studies.
Amplicon sequencing
Genome-wide association study
Next-generation sequencing
Plant microbiome
Rhizosphere
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
31
5
2022
pubmed:
1
6
2022
medline:
3
6
2022
Statut:
ppublish
Résumé
Plants form intimate associations with microorganisms, and these associations are directly impacted by the host genotype. However, identifying specific host genetic pathways that influence these microbial interactions has proved challenging. Genome-wide association-based approaches that use features of microbiome composition as a quantitative trait represent a novel and underutilized strategy to identify such pathways. Several recent studies have demonstrated the potential utility of plant microbiome-based genome-wide association studies (GWAS). In this chapter, we describe the process of implementing GWAS using the plant microbiome as the primary quantitative trait, considering experimental design, sample harvest, and processing, but with an emphasis on data filtering, data normalization, and statistical analyses.
Identifiants
pubmed: 35641774
doi: 10.1007/978-1-0716-2237-7_20
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
353-367Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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