Thienoguanosine, a unique non-perturbing reporter for investigating rotational dynamics of DNA duplexes and their complexes with proteins.

CCAAT-Enhancer-Binding Proteins / metabolism DNA / chemistry Fluorescence Polarization Guanosine / analogs & derivatives Ubiquitin-Protein Ligases / metabolism
Ab initio calculations DNA Fluorescence Fluorescent probes Time-resolved spectroscopy

Journal

International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578

Informations de publication

Date de publication:
31 Jul 2022
Historique:
received: 28 03 2022
revised: 02 05 2022
accepted: 23 05 2022
pubmed: 2 6 2022
medline: 24 6 2022
entrez: 1 6 2022
Statut: ppublish

Résumé

Time-resolved fluorescence anisotropy (TRFA) provides key information on the dynamics of biomolecules and their interaction with ligands. However, since natural nucleosides are almost non-fluorescent, its application to DNA duplexes (dsDNA) requires fluorescent labels, which can alter dsDNA stability, hinder protein binding, and complicate interpretation of TRFA experiments due to their local motion. As shown here, thienoguanosine (

Identifiants

DOI: 10.1016/j.ijbiomac.2022.05.162 PMID: 35643159
pubmed: 35643159
pii: S0141-8130(22)01146-1
doi: 10.1016/j.ijbiomac.2022.05.162
pii:
doi:

Substances chimiques

CCAAT-Enhancer-Binding Proteins 0
thienoguanosine 0
Guanosine 12133JR80S
DNA 9007-49-2
Ubiquitin-Protein Ligases EC 2.3.2.27

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

210-225

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Natalia Grytsyk (N)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France.

Ludovic Richert (L)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France.

Pascal Didier (P)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France.

Dmytro Dziuba (D)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France.

Stefano Ciaco (S)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France; Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.

Viola Mazzoleni (V)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France.

Thiebault Lequeu (T)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France.

Mattia Mori (M)

Department of Biotechnology, Chemistry and Pharmacy, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.

Yitzhak Tor (Y)

Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0358, USA.

Lara Martinez-Fernandez (L)

Departamento de Química, Facultad de Ciencias and Institute for Advanced Research in Chemistry (IADCHEM), Universidad Autónoma de Madrid, Campus de Excelencia UAM-CSIC, Cantoblanco, 28049 Madrid, Spain.

Roberto Improta (R)

Consiglio Nazionale delle Ricerche, Istituto Biostrutture e Bioimmagini, Via De Amicis 95, 80145 Napoli, Italy.

Yves Mély (Y)

Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de pharmacie, 74 route du Rhin, 67401 Illkirch, France. Electronic address: yves.mely@unistra.fr.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif basique et à glissière à leucines › Protéines liant les séquences stimulatrices de type CCAAT › Thienoguanosine, a unique non-perturbing...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Thienoguanosine, a unique non-perturbing...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Photométrie › Mesures de luminescence › Fluorimétrie › Polarisation de fluorescence › Thienoguanosine, a unique non-perturbing...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléosides › Ribonucléosides › Guanosine › Thienoguanosine, a unique non-perturbing...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Ligases › Ubiquitin-protein ligase complexes › Ubiquitin-protein ligases › Thienoguanosine, a unique non-perturbing...