Whole genome sequencing reveals hidden transmission of carbapenemase-producing Enterobacterales.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
01 06 2022
Historique:
received: 14 04 2021
accepted: 09 05 2022
entrez: 1 6 2022
pubmed: 2 6 2022
medline: 7 6 2022
Statut: epublish

Résumé

Carbapenemase-producing Enterobacterales (CPE) infection control practices are based on the paradigm that detected carriers in the hospital transmit to other patients who stay in the same ward. The role of plasmid-mediated transmission at population level remains largely unknown. In this retrospective cohort study over 4.7 years involving all multi-disciplinary public hospitals in Singapore, we analysed 779 patients who acquired CPE (1215 CPE isolates) detected by clinical or surveillance cultures. 42.0% met putative clonal transmission criteria, 44.8% met putative plasmid-mediated transmission criteria and 13.2% were unlinked. Only putative clonal transmissions associated with direct ward contact decreased in the second half of the study. Both putative clonal and plasmid-mediated transmission associated with indirect (no temporal overlap in patients' admission period) ward and hospital contact did not decrease during the study period. Indirect ward and hospital contact were identified as independent risk factors associated with clonal transmission. In conclusion, undetected CPE reservoirs continue to evade hospital infection prevention measures. New measures are needed to address plasmid-mediated transmission, which accounted for 50% of CPE dissemination.

Identifiants

pubmed: 35650193
doi: 10.1038/s41467-022-30637-5
pii: 10.1038/s41467-022-30637-5
pmc: PMC9160272
doi:

Substances chimiques

Bacterial Proteins 0
beta-Lactamases EC 3.5.2.6
carbapenemase EC 3.5.2.6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3052

Investigateurs

Kalisvar Marimuthu (K)
Indumathi Venkatachalam (I)
Benjamin Pei Zhi Cherng (BPZ)
Raymond Kok Choon Fong (RKC)
Surinder Kaur Pada (SK)
Say Tat Ooi (ST)
Nares Smitasin (N)
Koh Cheng Thoon (KC)
Li Yang Hsu (LY)
Tse Hsien Koh (TH)
Partha Pratim De (PP)
Thean Yen Tan (TY)
Douglas Chan (D)
Rama Narayana Deepak (RN)
Nancy Wen Sim Tee (NWS)
Michelle Ang (M)
Raymond Tzer Pin Lin (RTP)
Jeanette Teo (J)
Oon Tek Ng (OT)

Informations de copyright

© 2022. The Author(s).

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Auteurs

Kalisvar Marimuthu (K)

National Centre for Infectious Diseases, Singapore, Singapore. kalisvar_marimuthu@ttsh.com.sg.
Tan Tock Seng Hospital, Singapore, Singapore. kalisvar_marimuthu@ttsh.com.sg.
Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, Singapore, Singapore. kalisvar_marimuthu@ttsh.com.sg.

Indumathi Venkatachalam (I)

Singapore General Hospital, Singapore, Singapore.

Vanessa Koh (V)

National Centre for Infectious Diseases, Singapore, Singapore.
Tan Tock Seng Hospital, Singapore, Singapore.

Stephan Harbarth (S)

Infection Control Program, WHO Collaborating Center, Geneva University Hospitals and Faculty of Medicine, Geneva, Switzerland.

Eli Perencevich (E)

Departments of Internal Medicine and Epidemiology, University of Iowa Carver College of Medicine, Iowa City, USA.
Center for Comprehensive Access & Delivery Research and Evaluation, Iowa City Veterans Affairs Medical Center, Iowa City, USA.

Benjamin Pei Zhi Cherng (BPZ)

Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, Singapore, Singapore.
Singapore General Hospital, Singapore, Singapore.
DUKE-NUS Medical School, National University of Singapore, Singapore, Singapore.

Raymond Kok Choon Fong (RKC)

Changi General Hospital, Singapore, Singapore.

Surinder Kaur Pada (SK)

Ng Teng Fong General Hospital, Singapore, Singapore.

Say Tat Ooi (ST)

Khoo Teck Puat Hospital, Singapore, Singapore.

Nares Smitasin (N)

Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, Singapore, Singapore.
National University Hospital and National University Health System, Singapore, Singapore.

Koh Cheng Thoon (KC)

KK Women's and Children's Hospital, Singapore, Singapore.

Paul Anantharajah Tambyah (PA)

National University Hospital and National University Health System, Singapore, Singapore.

Li Yang Hsu (LY)

Yong Loo Lin School of Medicine, National University of Singapore and National University Health System, Singapore, Singapore.
Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore, Singapore.
National University of Singapore, Singapore, Singapore.

Tse Hsien Koh (TH)

Singapore General Hospital, Singapore, Singapore.
DUKE-NUS Medical School, National University of Singapore, Singapore, Singapore.

Partha Pratim De (PP)

Tan Tock Seng Hospital, Singapore, Singapore.

Thean Yen Tan (TY)

Changi General Hospital, Singapore, Singapore.

Douglas Chan (D)

Ng Teng Fong General Hospital, Singapore, Singapore.

Rama Narayana Deepak (RN)

Khoo Teck Puat Hospital, Singapore, Singapore.

Nancy Wen Sim Tee (NWS)

National University Hospital and National University Health System, Singapore, Singapore.

Andrea Kwa (A)

Singapore General Hospital, Singapore, Singapore.
Academic Clinical Programme (Medicine), SingHealth Duke-NUS, Singapore, Singapore.
Program in Emerging Infectious Diseases, Duke-NUS Medical School, Singapore, Singapore.

Yiying Cai (Y)

Singapore General Hospital, Singapore, Singapore.
National University of Singapore, Singapore, Singapore.

Yik-Ying Teo (YY)

Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore, Singapore.
National University of Singapore, Singapore, Singapore.

Natascha May Thevasagayam (NM)

National Centre for Infectious Diseases, Singapore, Singapore.
Tan Tock Seng Hospital, Singapore, Singapore.

Sai Rama Sridatta Prakki (SRS)

National Centre for Infectious Diseases, Singapore, Singapore.
Tan Tock Seng Hospital, Singapore, Singapore.

Weizhen Xu (W)

National Centre for Infectious Diseases, Singapore, Singapore.
Tan Tock Seng Hospital, Singapore, Singapore.

Wei Xin Khong (WX)

Tan Tock Seng Hospital, Singapore, Singapore.

David Henderson (D)

Clinical Centre, National Institutes of Health, Bethesda, USA.

Nicole Stoesser (N)

Nuffield Department of Medicine, University of Oxford, Oxford, UK.

David W Eyre (DW)

Nuffield Department of Population Health, University of Oxford, Oxford, UK.

Derrick Crook (D)

Nuffield Department of Medicine, University of Oxford, Oxford, UK.

Michelle Ang (M)

National Centre for Infectious Diseases, Singapore, Singapore.

Raymond Tzer Pin Lin (RTP)

National Centre for Infectious Diseases, Singapore, Singapore.
National University Hospital and National University Health System, Singapore, Singapore.

Angela Chow (A)

Tan Tock Seng Hospital, Singapore, Singapore.
Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore, Singapore.
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore.

Alex R Cook (AR)

Saw Swee Hock School of Public Health, National University of Singapore and National University Health System, Singapore, Singapore.

Jeanette Teo (J)

National University Hospital and National University Health System, Singapore, Singapore.

Oon Tek Ng (OT)

National Centre for Infectious Diseases, Singapore, Singapore. oon_tek_ng@ncid.sg.
Tan Tock Seng Hospital, Singapore, Singapore. oon_tek_ng@ncid.sg.
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore. oon_tek_ng@ncid.sg.

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Classifications MeSH