SAMBA: A Multicolor Digital Melting PCR Platform for Rapid Microbiome Profiling.
digital polymerase chain reactions
melt curve analyses
microbiomes
molecular probes
multiplexing
Journal
Small methods
ISSN: 2366-9608
Titre abrégé: Small Methods
Pays: Germany
ID NLM: 101724536
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
27
04
2022
received:
20
02
2022
pubmed:
3
6
2022
medline:
20
8
2022
entrez:
2
6
2022
Statut:
ppublish
Résumé
During the past decade, breakthroughs in sequencing technology have provided the basis for studies of the myriad ways in which microbial communities in and on the human body influence human health and disease. In almost every medical specialty, there is now a growing interest in accurate and quantitative profiling of the microbiota for use in diagnostic and therapeutic applications. However, the current next-generation sequencing approach for microbiome profiling is costly, requires laborious library preparation, and is challenging to scale up for routine diagnostics. Split, Amplify, and Melt analysis of BActeria-community (SAMBA), a novel multicolor digital melting polymerase chain reaction platform with unprecedented multiplexing capability is presented, and the capability to distinguish and quantify 16 bacteria species in mixtures is demonstrated. Subsequently, SAMBA is applied to measure the compositions of bacteria in the gut microbiome to identify microbial dysbiosis related to colorectal cancer. This rapid, low cost, and high-throughput approach will enable the implementation of microbiome diagnostics in clinical laboratories and routine medical practice.
Identifiants
pubmed: 35652511
doi: 10.1002/smtd.202200185
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200185Subventions
Organisme : National Medical Research Council
ID : NMRC/OFYIRG/0055/2017
Organisme : National Medical Research Council
ID : MOH-OFIRG18nov-0003
Informations de copyright
© 2022 Wiley-VCH GmbH.
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