Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2022
2022
Historique:
received:
12
01
2022
accepted:
18
05
2022
entrez:
3
6
2022
pubmed:
4
6
2022
medline:
9
6
2022
Statut:
epublish
Résumé
Live-cell microscopy imaging typically involves the use of high-quality glass-bottom chambers that allow cell culture, gaseous buffer exchange and optical properties suitable for microscopy applications. However, commercial sources of these chambers can add significant annual costs to cell biology laboratories. Consumer products in three-dimensional printing technology, for both Filament Deposition Modeling (FDM) and Masked Stereo Lithography (MSLA), have resulted in more biomedical research labs adopting the use of these devices for prototyping and manufacturing of lab plastic-based items, but rarely consumables. Here we describe a modular, live-cell chamber with multiple design options that can be mixed per experiment. Single reusable carriers and the use of biodegradable plastics, in a hybrid of FDM and MSLA manufacturing methods, reduce plastic waste. The system is easy to adapt to bespoke designs, with concept-to-prototype in a single day, offers significant cost savings to the users over commercial sources, and no loss in dimensional quality or reliability.
Identifiants
pubmed: 35657927
doi: 10.1371/journal.pone.0269345
pii: PONE-D-22-00858
pmc: PMC9165904
doi:
Substances chimiques
Plastics
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0269345Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Nat Commun. 2020 Nov 25;11(1):5979
pubmed: 33239615
J Biomater Sci Polym Ed. 1996;8(1):63-75
pubmed: 8933291
Molecules. 2021 Mar 31;26(7):
pubmed: 33807351
Biotechniques. 2002 Jan;32(1):80-2, 84, 86-7
pubmed: 11808703
J Microsc. 2022 Jan;285(1):29-39
pubmed: 34625963
Hum Mol Genet. 2017 Jan 15;26(2):395-406
pubmed: 28017939
Biochem Cell Biol. 2020 Oct;98(5):612-623
pubmed: 32339465
J Tissue Eng. 2017 Dec 21;8:2041731417744485
pubmed: 29318001
Sci Rep. 2017 Feb 10;7:42378
pubmed: 28186188
ACS Biomater Sci Eng. 2016 Oct 10;2(10):1732-1742
pubmed: 33440471
Mol Biol Cell. 2018 Nov 15;29(23):2809-2820
pubmed: 30256717
Commun Biol. 2021 Nov 4;4(1):1261
pubmed: 34737378
F1000Res. 2019 Nov 26;8:2003
pubmed: 32518624
Nat Cell Biol. 2018 Jul;20(7):755-765
pubmed: 29950571
Macromol Rapid Commun. 2012 Dec 13;33(23):2046-52
pubmed: 22987546
R Soc Open Sci. 2021 Nov 17;8(11):211158
pubmed: 34804575