Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads.
Immobilized
Ligand beads
Luciferase reporter assay
Notch signaling
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
8
6
2022
pubmed:
9
6
2022
medline:
11
6
2022
Statut:
ppublish
Résumé
Activation of Notch signaling requires physical interaction between ligand- and receptor-expressing cells and pulling force to release the Notch intracellular domain. Therefore, the soluble recombinant ligand protein is not suitable for the activation of Notch signaling in a cell culture system. Here, we describe an efficient method for transient activation of Notch signaling using immobilized ligand beads. Using this method, the timing of Notch signaling can be efficiently controlled.
Identifiants
pubmed: 35674892
doi: 10.1007/978-1-0716-2201-8_6
doi:
Substances chimiques
Ligands
0
Receptors, Notch
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
57-66Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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