Self-Assembled Multienzyme Nanostructures for Biocatalysis in Cellulo.
Biosynthesis
In cellulo
Multienzyme complexes
Protein assembly
Protein scaffold
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
10
6
2022
pubmed:
11
6
2022
medline:
15
6
2022
Statut:
ppublish
Résumé
Multienzyme complexes naturally exist in cells to catalyze cascade reactions in metabolic pathways. By clustering the enzymes in close proximity, these nanomachineries achieve effective conversion of metabolites. Bioengineers are working on the development of synthetic versions of multienzyme complexes in cells to synergize heterologous biosynthesis. Assembling enzymes on protein scaffolds through protein-protein interactions is a viable and facile way to form synthetic multienzyme complexes. Here, we describe the general methods to construct self-assembled multienzyme nanostructures in Escherichia coli for biosynthesis of valuable chemicals.
Identifiants
pubmed: 35687238
doi: 10.1007/978-1-0716-2269-8_13
doi:
Substances chimiques
Multienzyme Complexes
0
Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
197-204Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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