N-glycosylation as an effective strategy to enhance characteristics of Rhizomucor miehei lipase for biodiesel production.

Biofuels Glycosylation Lipase / metabolism Methanol / chemistry Rhizomucor / genetics
Biodiesel Loop structure Methanol tolerance N-glycosylation Rhizomucor miehei lipase

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Oct 2022
Historique:
received: 29 01 2022
revised: 27 05 2022
accepted: 30 05 2022
pubmed: 12 6 2022
medline: 10 8 2022
entrez: 11 6 2022
Statut: ppublish

Résumé

The construction of methanol-resistant lipases with high catalytic activity is world-shattering for biodiesel production. A semi-rational method has been constructed to enhance the properties of Rhizomucor miehei lipase with propeptide (ProRML) by introducing N-glycosylation sites in the Loop structure. The enzyme activities of the mutants N288 (1448.89 ± 68.64 U/mg) and N142 (1073.68 ± 33.87 U/mg) increased to 56.09 and 41.56 times relative to that of wild type ProRML (WT, 25.83 ± 0.73 U/mg), respectively. After incubation in 50 % methanol for 2.5 h, the residual activities of N314 and N174-1 were 95 % and 85%, which were higher than the WT (27 %). Additionally, the biodiesel yield of all mutants was increased after a one-time addition of methanol for 24 h. Among them, N288 increased the quantity of biodiesel from colza oil from 9.49 % to 88 %, and N314 increased the amount of biodiesel from waste soybean oil from 8.44% to 70%. This study provides an effective method to enhance the properties of lipase and improve its application potential in biodiesel production.

Identifiants

DOI: 10.1016/j.enzmictec.2022.110072 PMID: 35689964
pubmed: 35689964
pii: S0141-0229(22)00091-6
doi: 10.1016/j.enzmictec.2022.110072
pii:
doi:

Substances chimiques

Biofuels 0
Lipase EC 3.1.1.3
Methanol Y4S76JWI15

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110072

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Auteurs

Miao Tian (M)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

Zhiyuan Wang (Z)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China.

Junying Fu (J)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China.

Pengmei Lv (P)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China.

Cuiyi Liang (C)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China.

Zhibing Li (Z)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China.

LingMei Yang (L)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China.

Tao Liu (T)

Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou 510006, People's Republic of China.

Ming Li (M)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China; Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, People's Republic of China.

Wen Luo (W)

Key Laboratory of Renewable Energy, Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, People's Republic of China. Electronic address: luowen@ms.giec.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Environnement › Ressources de production d'énergie › Énergie renouvelable › Biocarburants › N-glycosylation as an effective strategy to...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Glycosylation › N-glycosylation as an effective strategy to...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Carboxylic ester hydrolases › Triacylglycerol lipase › N-glycosylation as an effective strategy to...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Méthanol › N-glycosylation as an effective strategy to...
questionsmedicales.fr › Eucaryotes › Champignons › Champignons non classés › Mucorales › Rhizomucor › N-glycosylation as an effective strategy to...