Investigation of fungal contamination in medicinal and edible Lycii Fructus through DNA metabarcoding.
DNA metabarcoding
ITS2
Lycii Fructus
collection areas
fungi
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Sep 2022
Sep 2022
Historique:
revised:
09
05
2022
received:
08
09
2021
accepted:
06
06
2022
pubmed:
14
6
2022
medline:
8
9
2022
entrez:
13
6
2022
Statut:
ppublish
Résumé
Lycii Fructus (LF) is considered as a 'superfood' due to its health benefits and delicious tastes, which has gained popularity worldwide. However, LF is also a proper host for fungal growth due to its abundant nutrients. Fungal contamination seriously affects the quality and safety of LF and poses threats to consumer health. In this study, a total of 15 LF samples were collected from five provinces in China, and were divided into five groups based on the collection areas. Fungal contamination in LF was investigated by targeting the internal transcribed spacer 2 region using Illumina Miseq PE300 platform, and the differences of fungal community in groups based on collection areas were compared. Results showed that the fungal contamination was detected in all the 15 LF samples. Ascomycota, Dothideomycetes, Pleosporales and Pleosporaceae were dominant at the phylum, class, order and family levels, respectively. At the genus level, Alternaria, Cladosporium and Fusarium were the three dominant genera. In all, 24 fungal species were identified. Among which, two species, namely Penicillium oxalicum and Trichothecium roseum, were potentially toxigenic. All 15 LF samples were detected with fungal contamination. The differences of fungal community in LF samples collected from different areas were observed. DNA metabarcoding was demonstrated as an efficient method to monitor the fungal contamination in LF. This work comprehensively reveals the fungal diversity and composition in LF and provides early warning for potential mycotoxin contamination.
Substances chimiques
DNA, Fungal
0
Mycotoxins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1555-1565Subventions
Organisme : National Key R&D Program of China
ID : 2019YFC1711100
Organisme : CAMS Innovation Fund for Medical Sciences (CIFMS)
ID : 2021-I2M-1-071
Informations de copyright
© 2022 Society for Applied Microbiology.
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