Cryo-EM structures of pentameric autoinducer-2 exporter from Escherichia coli reveal its transport mechanism.
TqsA
YdiK
autoinducer-2
pentamer
quorum sensing
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
15 09 2022
15 09 2022
Historique:
revised:
04
05
2022
received:
20
10
2021
accepted:
06
05
2022
pubmed:
15
6
2022
medline:
17
9
2022
entrez:
14
6
2022
Statut:
ppublish
Résumé
Bacteria utilize small extracellular molecules to communicate in order to collectively coordinate their behaviors in response to the population density. Autoinducer-2 (AI-2), a universal molecule for both intra- and inter-species communication, is involved in the regulation of biofilm formation, virulence, motility, chemotaxis, and antibiotic resistance. While many studies have been devoted to understanding the biosynthesis and sensing of AI-2, very little information is available on its export. The protein TqsA from Escherichia coli, which belongs to the AI-2 exporter superfamily, has been shown to export AI-2. Here, we report the cryogenic electron microscopic structures of two AI-2 exporters (TqsA and YdiK) from E. coli at 3.35 Å and 2.80 Å resolutions, respectively. Our structures suggest that the AI-2 exporter exists as a homo-pentameric complex. In silico molecular docking and native mass spectrometry experiments were employed to demonstrate the interaction between AI-2 and TqsA, and the results highlight the functional importance of two helical hairpins in substrate binding. We propose that each monomer works as an independent functional unit utilizing an elevator-type transport mechanism.
Identifiants
pubmed: 35698912
doi: 10.15252/embj.2021109990
pmc: PMC9475539
doi:
Substances chimiques
Bacterial Proteins
0
Lactones
0
N-octanoylhomoserine lactone
0
Homoserine
6KA95X0IVO
Banques de données
PDB
['7NB6', '7OT9']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e109990Informations de copyright
© 2022 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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