Druggability Evaluation of the Neuron Derived Orphan Receptor (NOR-1) Reveals Inverse NOR-1 Agonists.

NR4A3 fragment screening neurodegeneration nuclear receptor transcription factor

Journal

ChemMedChem
ISSN: 1860-7187
Titre abrégé: ChemMedChem
Pays: Germany
ID NLM: 101259013

Informations de publication

Date de publication:
17 08 2022
Historique:
received: 09 05 2022
pubmed: 16 6 2022
medline: 20 8 2022
entrez: 15 6 2022
Statut: ppublish

Résumé

The neuron derived orphan receptor (NOR-1, NR4A3) is among the least studied nuclear receptors. Its physiological role and therapeutic potential remain widely elusive which is in part due to the lack of chemical tools that can directly modulate NOR-1 activity. To probe the possibility of pharmacological NOR-1 modulation, we have tested a drug fragment library for NOR-1 activation and repression. Despite low hit-rate (<1 %), we have obtained three NOR-1 ligand chemotypes one of which could be rapidly expanded to an analogue comprising low micromolar inverse NOR-1 agonist potency and altering NOR-1 regulated gene expression in a cellular setting. It confirms druggability of the transcription factor and may serve as an early tool to assess the role and potential of NOR-1.

Identifiants

pubmed: 35704774
doi: 10.1002/cmdc.202200259
pmc: PMC9542104
doi:

Substances chimiques

Ligands 0
Receptors, Cytoplasmic and Nuclear 0
Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202200259

Informations de copyright

© 2022 The Authors. ChemMedChem published by Wiley-VCH GmbH.

Références

Sci Rep. 2020 Feb 18;10(1):2851
pubmed: 32071334
J Steroid Biochem Mol Biol. 2016 Mar;157:48-60
pubmed: 25917081
Methods Mol Biol. 2019;1966:175-192
pubmed: 31041747
J Pharmacol Exp Ther. 2005 Apr;313(1):460-73
pubmed: 15615863
J Med Chem. 2021 Mar 11;64(5):2659-2668
pubmed: 33629841
Mol Cell Biol. 2015 Jul;35(14):2530-40
pubmed: 25963659
Nature. 1988 Oct 6;335(6190):563-4
pubmed: 3047590
J Biol Chem. 2021 Jul;297(1):100814
pubmed: 34081964
Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12317-22
pubmed: 20566846
ChemMedChem. 2022 Aug 17;17(16):e202200259
pubmed: 35704774
Mol Cell Endocrinol. 2009 Oct 15;309(1-2):101-8
pubmed: 19465082
J Med Chem. 1996 Jul 19;39(15):2887-93
pubmed: 8709122
J Biol Chem. 2003 Jul 4;278(27):24776-90
pubmed: 12709428
ChemMedChem. 2021 Apr 8;16(7):1088-1092
pubmed: 33283450
J Med Chem. 2021 Jun 24;64(12):8727-8738
pubmed: 34115934
ACS Pharmacol Transl Sci. 2021 Nov 23;4(6):1794-1807
pubmed: 34927011
Adv Sci (Weinh). 2022 Jun;9(18):e2104640
pubmed: 35488520
J Neurosurg. 2016 Jun;124(6):1654-64
pubmed: 26613168
Mol Cell Biol. 2004 Oct;24(20):9070-8
pubmed: 15456880
Sci Rep. 2016 May 16;6:25944
pubmed: 27181368
PLoS One. 2016 Mar 03;11(3):e0150450
pubmed: 26938745
Int J Mol Sci. 2020 Oct 24;21(21):
pubmed: 33114319
J Med Chem. 2021 Jul 22;64(14):9592-9638
pubmed: 34251209
Neuropathology. 2015 Dec;35(6):503-9
pubmed: 26096603
Biol Pharm Bull. 2005 Sep;28(9):1603-7
pubmed: 16141523
J Med Chem. 2021 Oct 28;64(20):15126-15140
pubmed: 34633810
Oncogenesis. 2012 Jul 02;1:e19
pubmed: 23552735
J Med Chem. 2022 Feb 10;65(3):2288-2296
pubmed: 34989568

Auteurs

Daniel Zaienne (D)

Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.

Silvia Arifi (S)

Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.

Julian A Marschner (JA)

Department of Pharmacy, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377, Munich, Germany.

Jan Heering (J)

Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Theodor-Stern-Kai 7, 60596, Frankfurt, Germany.

Daniel Merk (D)

Department of Pharmacy, Ludwig-Maximilians-Universität München, Butenandtstr. 5-13, 81377, Munich, Germany.
Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Max-von-Laue-Str. 9, 60438, Frankfurt, Germany.

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Classifications MeSH