Immobilization of urease enzyme on chitosan/polyvinyl alcohol electrospun nanofibers.
biocatalyst
electrospinning
electrospun nanofiber
enzyme immobilization
urease
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
09 2022
09 2022
Historique:
revised:
30
04
2022
received:
07
04
2020
accepted:
29
05
2022
pubmed:
17
6
2022
medline:
15
10
2022
entrez:
16
6
2022
Statut:
ppublish
Résumé
Electrospun nanofibers have gained much attention for enzyme immobilization due to their high surface-to-volume ratio. In this study, urease was immobilized on chitosan/poly(vinyl alcohol) (PVA) nanofibers by both adsorption and crosslinking methods. In order to obtain nanofibers with more desirable properties, solutions with different ratios of chitosan and PVA were electrospun and crosslinked using glutaraldehyde. Comparing SEM images of the nanofibers, before and after immersing them in phosphate buffer, it was shown that higher chitosan content leads to more stable fibers. So, the solution with the chitosan to PVA ratio of 40:60 was used for enzyme immobilization. Then, the effects of initial protein concentration, temperature, incubation time, and method of immobilization were investigated to reach the highest enzyme activity. Under similar immobilization conditions, covalently immobilized urease showed higher activity, compared to uncrosslinked immobilized enzyme. Besides, it retained 30% of its initial activity after 10 times usage. So, this method was chosen for further investigation. Not only the activity of the immobilized enzyme was much higher than the free enzyme in a wide range of pH and temperature, but also stability of the immobilized enzyme was improved. Immobilized urease was then used to remove thiourea which is a toxic compound. Findings indicated 60% hydrolysis of initial thiourea in 12 h. In conclusion, the findings showed that chitosan/PVA nanofibers are suitable candidates for the immobilization of urease.
Substances chimiques
Enzymes, Immobilized
0
Phosphates
0
Polyvinyl Alcohol
9002-89-5
Chitosan
9012-76-4
Urease
EC 3.5.1.5
Thiourea
GYV9AM2QAG
Glutaral
T3C89M417N
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3282Informations de copyright
© 2022 American Institute of Chemical Engineers.
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