OzTracs: Optical Osmolality Reporters Engineered from Mechanosensitive Ion Channels.
mechanosensitive protein
osmolality
sensor
yeast
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
04 06 2022
04 06 2022
Historique:
received:
26
04
2022
revised:
18
05
2022
accepted:
02
06
2022
entrez:
24
6
2022
pubmed:
25
6
2022
medline:
28
6
2022
Statut:
epublish
Résumé
Interactions between physical forces and membrane proteins underpin many forms of environmental sensation and acclimation. Microbes survive osmotic stresses with the help of mechanically gated ion channels and osmolyte transporters. Plant mechanosensitive ion channels have been shown to function in defense signaling. Here, we engineered genetically encoded osmolality sensors (OzTracs) by fusing fluorescent protein spectral variants to the mechanosensitive ion channels MscL from
Identifiants
pubmed: 35740912
pii: biom12060787
doi: 10.3390/biom12060787
pmc: PMC9221499
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
Escherichia coli Proteins
0
Ion Channels
0
MSL10 protein, Arabidopsis
0
Membrane Proteins
0
MscL protein, E coli
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : 2R01GM084211
Pays : United States
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