Insights into the mechanism of action of the arbitrium communication system in SPbeta phages.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 06 2022
24 06 2022
Historique:
received:
20
04
2021
accepted:
01
06
2022
pubmed:
25
6
2022
medline:
29
6
2022
entrez:
24
6
2022
Statut:
epublish
Résumé
The arbitrium system is employed by phages of the SPbeta family to communicate with their progeny during infection to decide either to follow the lytic or the lysogenic cycle. The system is controlled by a peptide, AimP, that binds to the regulator AimR, inhibiting its DNA-binding activity and expression of aimX. Although the structure of AimR has been elucidated for phages SPβ and phi3T, there is still controversy regarding the molecular mechanism of AimR function, with two different proposed models for SPβ. In this study, we deepen our understanding of the system by solving the structure of an additional AimR that shows chimerical characteristics with the SPβ receptor. The crystal structures of this AimR (apo, AimP-bound and DNA-bound) together with in vitro and in vivo analyses confirm a mechanism of action by AimP-induced conformational restriction, shedding light on peptide specificity and cross regulation with relevant biological implications.
Identifiants
pubmed: 35750663
doi: 10.1038/s41467-022-31144-3
pii: 10.1038/s41467-022-31144-3
pmc: PMC9232636
doi:
Substances chimiques
Peptides
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3627Subventions
Organisme : Medical Research Council
ID : MR/M003876/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V000772/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/V002376/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 201531/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S00940X/2
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N002873/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S003835/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/S00940X/1
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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