Protective effect of the stressed supernatant from Lactococcus lactis subsp. lactis and its metabolic analysis.
Dithiothreitol
Lactococcus lactis subsp. lactis
Protective effect
Trehalose
UV stress
Journal
Archives of microbiology
ISSN: 1432-072X
Titre abrégé: Arch Microbiol
Pays: Germany
ID NLM: 0410427
Informations de publication
Date de publication:
25 Jun 2022
25 Jun 2022
Historique:
received:
07
01
2022
accepted:
01
06
2022
revised:
29
05
2022
entrez:
25
6
2022
pubmed:
26
6
2022
medline:
29
6
2022
Statut:
epublish
Résumé
There are numerous factors restricting wide application of lactic acid bacteria (LAB) in dairy industry, causing urgent demands for novel bioprotectants. Protective effects and metabolites of Lactococcus lactis subsp. lactis (L. lactis) from ultraviolet (UV)-induced supernatant were investigated and the protective mechanism was explored. The strain viability of the group treated with the supernatant of continuous UV irradiation (V1) and the group with intermittent UV irradiation (V2) was 8.45 and 14.13 times of the control group, respectively. Further exploration on the protective of L. lactis supernatant, under different dose of UV treatment, showed it was dose-dependent. The condition for the supernatant with best protective effect was vertical distance 50.00 cm, horizontal distance 25.00 cm, intermittent UV irradiation (30 s interval 30 s) for 4.5 min (V2), which was chose for untargeted metabolite analysis. And that in V1 was for comparative study. There were 181 up-regulated metabolites in V1 and 161 up-regulated metabolites in V2, respectively. Most of the up-regulated metabolites were related to secondary metabolite synthesis, environmental microbial metabolism, antibiotic synthesis and amino acid biosynthesis. Notably, production of dithiothreitol (DTT) in V2 was 65.2-fold higher than that in the control group. Trehalose in ABC transporter pathway was also up-regulated in the metabolites induced by UV. Results indicated that L. lactis could adapt to the UV stress by adjusting metabolic pathways and producing special metabolites to protect itself. This research offers the basis for robust strain development and contributes to initial study on potential bioprotectant.
Identifiants
pubmed: 35751720
doi: 10.1007/s00203-022-03034-1
pii: 10.1007/s00203-022-03034-1
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
428Subventions
Organisme : the National Natural Science Foundation of China
ID : 81870093
Organisme : the National Natural Science Foundation of China
ID : 31900296
Organisme : the Research Project of People's Liberation Army
ID : BX115C007
Organisme : the Research Project of People's Liberation Army
ID : BFP20C006
Organisme : Natural Science Foundation of Henan Province
ID : 202300410365
Organisme : Key Scientific Research Projects of Higher Education Institutions in Henan Province
ID : 21A530006
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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