Integrated metabolomics and transcriptome analysis on flavonoid biosynthesis in flowers of safflower (
Colour-transition
Flavonoid glycosides biosythesis
Glycosyltransferases
Metabolomics
Safflower
Transcriptome
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2022
2022
Historique:
received:
08
03
2022
accepted:
24
05
2022
entrez:
28
6
2022
pubmed:
29
6
2022
medline:
29
6
2022
Statut:
epublish
Résumé
Safflower ( In this research, widely targeted metabolomics and transcriptomics were used to elucidate the changes in flavonoid biosynthesis from the gene and chemical points of view in flowers of safflower during colour transition. The screening of differential metabolites depended on fold change and variable importance in project (VIP) value. Differential expressed genes (DEGs) were screened by DESeq2 method. RT-PCR was used to analyse relative expressions of DEGs. A total of 212 flavonoid metabolites, including hydroxysafflor yellow A, carthamin and anthocyanins, were detected and showed a large difference. The candidate genes of glycosyltransferases and flavonoid hydroxylase that might participate in flavonoid glycoside biosynthesis were screened. Ten candidate genes were screened. Through integrated metabolomics and transcriptome analysis, a uridine diphosphate glucose glycosyltransferase gene,
Sections du résumé
Background
Safflower (
Methods
In this research, widely targeted metabolomics and transcriptomics were used to elucidate the changes in flavonoid biosynthesis from the gene and chemical points of view in flowers of safflower during colour transition. The screening of differential metabolites depended on fold change and variable importance in project (VIP) value. Differential expressed genes (DEGs) were screened by DESeq2 method. RT-PCR was used to analyse relative expressions of DEGs.
Results
A total of 212 flavonoid metabolites, including hydroxysafflor yellow A, carthamin and anthocyanins, were detected and showed a large difference. The candidate genes of glycosyltransferases and flavonoid hydroxylase that might participate in flavonoid glycoside biosynthesis were screened. Ten candidate genes were screened. Through integrated metabolomics and transcriptome analysis, a uridine diphosphate glucose glycosyltransferase gene,
Identifiants
pubmed: 35762018
doi: 10.7717/peerj.13591
pii: 13591
pmc: PMC9233481
doi:
Substances chimiques
Anthocyanins
0
Flavonoids
0
Glycosides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
e13591Informations de copyright
© 2022 Ren et al.
Déclaration de conflit d'intérêts
The authors declare that they have no competing interests.
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