Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging.

Fluorescence microscopy Mitochondria membrane potential Primary skin fibroblasts TMRM Uncoupler

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 30 6 2022
pubmed: 1 7 2022
medline: 6 7 2022
Statut: ppublish

Résumé

The mitochondrial membrane potential (ΔΨ

Identifiants

pubmed: 35771453
doi: 10.1007/978-1-0716-2309-1_22
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

319-324

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

Briston T, Roberts M, Lewis S, Powney B, Staddon JM, Szabadkai G, Duchen MR (2017) Mitochondrial permeability transition pore: sensitivity to opening and mechanistic dependence on substrate availability. Sci Rep 7(1):10492
doi: 10.1038/s41598-017-10673-8
Duchen MR (2004) Mitochondria in health and disease: perspectives on a new mitochondrial biology. Mol Asp Med 25(4):365–451
doi: 10.1016/j.mam.2004.03.001
Bazhin AA, Sinisi R, De Marchi U, Hermant A, Sambiagio N, Maric T, Budin G, Goun EA (2020) A bioluminescent probe for longitudinal monitoring of mitochondrial membrane potential. Nat Chem Biol 16(12):1385–1393
doi: 10.1038/s41589-020-0602-1
Wacquier B, Combettes L, Dupont G (2020) Dual dynamics of mitochondrial permeability transition pore opening. Sci Rep 10(1):3924
doi: 10.1038/s41598-020-60177-1
Bhosale G, Duchen MR (2019) Investigating the mitochondrial permeability transition pore in disease phenotypes and drug screening. Curr Protoc Pharmacol 85(1):e59
doi: 10.1002/cpph.59
Granatiero V, Pacifici M, Raffaello A, De Stefani D, Rizzuto R (2019) Overexpression of mitochondrial calcium uniporter causes neuronal death. Oxidative Med Cell Longev 2019:1681254
doi: 10.1155/2019/1681254
Duchen MR, Surin A, Jacobson J (2003) Imaging mitochondrial function in intact cells. Methods Enzymol 361:353–389
doi: 10.1016/S0076-6879(03)61019-0

Auteurs

Gabriel Esteban Valdebenito (GE)

Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK.

Michael R Duchen (MR)

Department of Cell and Developmental Biology and Consortium for Mitochondrial Research, UCL, London, UK. m.duchen@ucl.ac.uk.

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Classifications MeSH