Kinase expression enhances phenolic aldehydes conversion and ethanol fermentability of Zymomonas mobilis.

Aldehydes / metabolism Ethanol / metabolism Fermentation Zymomonas / genetics
Ethanol fermentation Kinase Phenolic aldehyde inhibitors ZMO1162 Zymomonas mobilis ZM4

Journal

Bioprocess and biosystems engineering
ISSN: 1615-7605
Titre abrégé: Bioprocess Biosyst Eng
Pays: Germany
ID NLM: 101088505

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 28 03 2022
accepted: 11 06 2022
pubmed: 6 7 2022
medline: 12 8 2022
entrez: 5 7 2022
Statut: ppublish

Résumé

Kinases modulate the various physiological activities of microbial fermenting strains including the conversion of lignocellulose-derived phenolic aldehydes (4-hydroxyaldehyde, vanillin, and syringaldehyde). Here, we comprehensively investigated the gene transcriptional profiling of the kinases under the stress of phenolic aldehydes for ethanologenic Zymomonas mobilis using DNA microarray. Among 47 kinase genes, three genes of ZMO0003 (adenylylsulfate kinase), ZMO1162 (histidine kinase), and ZMO1391 (diacylglycerol kinase), were differentially expressed against 4-hydroxybenzaldehyde and vanillin, in which the overexpression of ZMO1162 promoted the phenolic aldehydes conversion and ethanol fermentability. The perturbance originated from plasmid-based expression of ZMO1162 gene contributed to a unique expression profiling of genome-encoding genes under all three phenolic aldehydes stress. Differentially expressed ribosome genes were predicted as one of the main contributors to phenolic aldehydes conversion and thus finally enhanced ethanol fermentability for Z. mobilis ZM4. The results provided an insight into the kinases on regulation of phenolic aldehydes conversion and ethanol fermentability for Z. mobilis ZM4, as well as the target object for rational design of robust biorefinery strains.

Identifiants

DOI: 10.1007/s00449-022-02747-3 PMID: 35786774
pubmed: 35786774
doi: 10.1007/s00449-022-02747-3
pii: 10.1007/s00449-022-02747-3
doi:

Substances chimiques

Aldehydes 0
Ethanol 3K9958V90M

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1319-1329

Subventions

Organisme : Base and talent project-high level science-innovation talent of Jiujiang Science and Technology Bureau
ID : S2021QNZZ030

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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Auteurs

Xia Yi (X)

Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, Jiujiang, 332000, China. yixia@mail.ecust.edu.cn.
National-Local Joint Engineering Research Center for Biomass Refining and High-Quality Utilization, Changzhou University, Changzhou, 213164, China. yixia@mail.ecust.edu.cn.
ORCID: 0000-0002-0260-4057

Jianfang Wu (J)

Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, Jiujiang, 332000, China.

He Jiang (H)

Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, Jiujiang, 332000, China.

Yan Zhao (Y)

Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, Jiujiang, 332000, China.

Jun Mei (J)

Jiangxi Provincial Key Laboratory of Systems Biomedicine, Jiujiang University, 17 Lufeng Road, Jiujiang, 332000, China.

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Classifications MeSH

questionsmedicales.fr › Composés chimiques organiques › Aldéhydes › Kinase expression enhances phenolic aldehydes...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Éthanol › Kinase expression enhances phenolic aldehydes...
questionsmedicales.fr › Métabolisme › Métabolisme glucidique › Fermentation › Kinase expression enhances phenolic aldehydes...
questionsmedicales.fr › Bactéries › Proteobacteria › Alphaproteobacteria › Sphingomonadaceae › Zymomonas › Kinase expression enhances phenolic aldehydes...