Characterization of the urogenital microbiome in Miniature Schnauzers with and without calcium oxalate urolithiasis.
canine
stones
urinary tract infection
urobiome
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
received:
24
01
2022
accepted:
15
06
2022
pubmed:
8
7
2022
medline:
27
7
2022
entrez:
7
7
2022
Statut:
ppublish
Résumé
Calcium oxalate (CaOx) uroliths are common in dogs. Humans with CaOx urolithiasis exhibit alterations of the urinary and urogenital microbiomes that might mediate urolith formation. Detection of urogenital microbes associated with CaOx in dogs could inform disease pathophysiology. To identify compositional differences in the urogenital microbiome of Miniature Schnauzers with and without CaOx uroliths. Nineteen midstream, voided urine samples from Miniature Schnauzers with (n = 9) and without (n = 10) a history of CaOx urolithiasis. Analytical cross-sectional study. Microbial DNA was extracted from previously frozen urine samples and sequenced for the bacterial 16S rRNA V3-V4 hypervariable regions. Diversity and composition of microbial populations were compared between urolith formers and controls. Alpha and beta diversity measures were similar between groups. Five individual bacterial taxa differed in abundance (indicator values >0.5 and P < .05): Acinetobacter, 2 Geobacillus variants, and Hydrogenophaga were overrepresented in the urine of urolith formers, and Sphingopyxis was overrepresented in controls. Two distinct subtypes of urine microbial composition were observed based on beta diversity measures, independent of urolith status, and other clinical variables. Although we did not detect a difference in the overall urogenital microbial composition between groups, observed differences in individual bacterial taxa might be clinically relevant. For example, Acinetobacter was overrepresented in urolith formers and is associated with CaOx urolithiasis in humans. Two unique clusters of the microbiome were identified, independent of urolith status, which may represent distinct urotypes present in Miniature Schnauzers.
Sections du résumé
BACKGROUND
BACKGROUND
Calcium oxalate (CaOx) uroliths are common in dogs. Humans with CaOx urolithiasis exhibit alterations of the urinary and urogenital microbiomes that might mediate urolith formation. Detection of urogenital microbes associated with CaOx in dogs could inform disease pathophysiology.
OBJECTIVE
OBJECTIVE
To identify compositional differences in the urogenital microbiome of Miniature Schnauzers with and without CaOx uroliths.
ANIMALS
METHODS
Nineteen midstream, voided urine samples from Miniature Schnauzers with (n = 9) and without (n = 10) a history of CaOx urolithiasis.
METHODS
METHODS
Analytical cross-sectional study. Microbial DNA was extracted from previously frozen urine samples and sequenced for the bacterial 16S rRNA V3-V4 hypervariable regions. Diversity and composition of microbial populations were compared between urolith formers and controls.
RESULTS
RESULTS
Alpha and beta diversity measures were similar between groups. Five individual bacterial taxa differed in abundance (indicator values >0.5 and P < .05): Acinetobacter, 2 Geobacillus variants, and Hydrogenophaga were overrepresented in the urine of urolith formers, and Sphingopyxis was overrepresented in controls. Two distinct subtypes of urine microbial composition were observed based on beta diversity measures, independent of urolith status, and other clinical variables.
CONCLUSIONS AND CLINICAL IMPORTANCE
CONCLUSIONS
Although we did not detect a difference in the overall urogenital microbial composition between groups, observed differences in individual bacterial taxa might be clinically relevant. For example, Acinetobacter was overrepresented in urolith formers and is associated with CaOx urolithiasis in humans. Two unique clusters of the microbiome were identified, independent of urolith status, which may represent distinct urotypes present in Miniature Schnauzers.
Identifiants
pubmed: 35796316
doi: 10.1111/jvim.16482
pmc: PMC9308445
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Calcium Oxalate
2612HC57YE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1341-1352Subventions
Organisme : University of Minnesota's College of Veterinary Medicine Resident and Graduate Student Research Grant
Organisme : NIH HHS
ID : T32 OD010993
Pays : United States
Organisme : NIH HHS
ID : K01 OD019912
Pays : United States
Organisme : NIH ORIP K01 Mentored Research Scientist Development Award
ID : K01-OD019912
Organisme : NIH T32 Comparative Medicine and Pathology Training Grant
ID : T32 OD010993-15
Informations de copyright
© 2022 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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