Blocking of SGLT2 to Eliminate NADPH-Induced Oxidative Stress in Lenses of Animals with Fructose-Induced Diabetes Mellitus.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
27 Jun 2022
Historique:
received: 25 05 2022
revised: 20 06 2022
accepted: 24 06 2022
entrez: 9 7 2022
pubmed: 10 7 2022
medline: 14 7 2022
Statut: epublish

Résumé

Chronic hyperglycemia triggers an abnormal rise in reactive oxygen species (ROS) that leads to blindness in patients with diabetes mellitus (DM) and cataracts. In this study, the effects of dapagliflozin, metformin and resveratrol on ROS production were investigated in lens epithelial cells (LECs) of animals with fructose-induced DM. LECs were isolated from patients without DM, or with DM devoid of diabetic retinopathy. Animals were treated with 10% fructose for 8 weeks to induce DM, which was verified by monitoring blood pressure and serum parameters. For drug treatments, 1.2 mg/day of dapagliflozin was given for 2 weeks, 500 mg/kg/day of metformin was given, and 10 mg/kg/day of resveratrol was given. Dihydroethidium was used to stain endogenous O

Identifiants

pubmed: 35806147
pii: ijms23137142
doi: 10.3390/ijms23137142
pmc: PMC9266761
pii:
doi:

Substances chimiques

Reactive Oxygen Species 0
Receptor for Advanced Glycation End Products 0
Sodium-Glucose Transporter 2 0
Fructose 30237-26-4
NADP 53-59-8
Metformin 9100L32L2N
NADPH Oxidases EC 1.6.3.-
Resveratrol Q369O8926L

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Kaohsiung Veterans General Hospital
ID : KSVGH110-141

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Auteurs

Ying-Ying Chen (YY)

Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.
School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan.

Tsung-Tien Wu (TT)

Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.
School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan.

Chiu-Yi Ho (CY)

Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.
Department of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.

Tung-Chen Yeh (TC)

Department of Internal Medicine, Division of Cardiology, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.

Gwo-Ching Sun (GC)

Department of Anesthesiology, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.

Ching-Jiunn Tseng (CJ)

Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 40402, Taiwan.

Pei-Wen Cheng (PW)

Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81362, Taiwan.
Department of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.

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