Structural basis of the bHLH domains of MyoD-E47 heterodimer.

DNA-Binding Proteins / metabolism Helix-Loop-Helix Motifs MyoD Protein / metabolism Protein Binding TCF Transcription Factors / metabolism Transcription Factor 7-Like 1 Protein / metabolism Transcription Factors / metabolism
Crystal structure E47 Heterodimer MyoD bHLH domain

Journal

Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516

Informations de publication

Date de publication:
17 09 2022
Historique:
received: 05 06 2022
revised: 15 06 2022
accepted: 22 06 2022
pubmed: 11 7 2022
medline: 6 8 2022
entrez: 10 7 2022
Statut: ppublish

Résumé

The basic helix-loop-helix (bHLH) family is one of the most conserved transcription factor families that plays an important role in regulating cell growth, differentiation and tissue development. Typically, members of this family form homo- or heterodimers to recognize specific motifs and activate transcription. MyoD is a vital transcription factor that regulates muscle cell differentiation. However, it is necessary for MyoD to form a heterodimer with E-proteins to activate transcription. Even though the crystal structure of the MyoD homodimer has been determined, the structure of the MyoD heterodimer in complex with the E-box protein remains unclear. In this study, we determined the crystal structure of the bHLH domain of the MyoD-E47 heterodimer at 2.05 Å. Our structural analysis revealed that MyoD interacts with E47 through a hydrophobic interface. Moreover, we confirmed that heterodimerization could enhance the binding affinity of MyoD to E-box sequences. Our results provide new structural insights into the heterodimer of MyoD and E-box protein, suggesting the molecular mechanism of transcription activation of MyoD upon binding to E-box protein.

Identifiants

DOI: 10.1016/j.bbrc.2022.06.071 PMID: 35810596
pubmed: 35810596
pii: S0006-291X(22)00925-1
doi: 10.1016/j.bbrc.2022.06.071
pii:
doi:

Substances chimiques

DNA-Binding Proteins 0
MyoD Protein 0
TCF Transcription Factors 0
Transcription Factor 7-Like 1 Protein 0
Transcription Factors 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

88-93

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Auteurs

Jiayun Zhong (J)

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, 200438, Shanghai, China.

Zhaohui Jin (Z)

Department of General Surgery, Shanghai Key Laboratory of Biliary Tract Disease Research, State Key Laboratory of Oncogenes and Related Genes, Xinhua Hospital, Shanghai Jiao Tong University, 200092, Shanghai, China.

Lin Jiang (L)

Department of General Surgery, Shanghai Key Laboratory of Biliary Tract Disease Research, State Key Laboratory of Oncogenes and Related Genes, Xinhua Hospital, Shanghai Jiao Tong University, 200092, Shanghai, China.

Lingxiao Zhang (L)

Department of Biliary-Pancreatic Surgery, Shanghai Key Laboratory of Biliary Tract Disease Research, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, Shanghai Jiao Tong University, 200120, Shanghai, China.

Zetao Hu (Z)

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, 200438, Shanghai, China.

Yuhan Zhang (Y)

Department of General Surgery, Shanghai Key Laboratory of Biliary Tract Disease Research, State Key Laboratory of Oncogenes and Related Genes, Xinhua Hospital, Shanghai Jiao Tong University, 200092, Shanghai, China.

Yingbin Liu (Y)

Department of Biliary-Pancreatic Surgery, Shanghai Key Laboratory of Biliary Tract Disease Research, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, Shanghai Jiao Tong University, 200120, Shanghai, China.

Jinbiao Ma (J)

State Key Laboratory of Genetic Engineering, Collaborative Innovation Center of Genetics and Development, Department of Biochemistry, Institute of Plant Biology, School of Life Sciences, Fudan University, 200438, Shanghai, China. Electronic address: majb@fudan.edu.cn.

Ying Huang (Y)

Department of General Surgery, Shanghai Key Laboratory of Biliary Tract Disease Research, State Key Laboratory of Oncogenes and Related Genes, Xinhua Hospital, Shanghai Jiao Tong University, 200092, Shanghai, China. Electronic address: huangying@xinhuamed.com.cn.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Structural basis of the bHLH domains of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Motifs à hélice-boucle-hélice › Structural basis of the bHLH domains of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription à motif basique hélice-boucle-hélice › Facteurs de régulation myogènes › Protéine MyoD › Structural basis of the bHLH domains of...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Structural basis of the bHLH domains of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription TCF › Structural basis of the bHLH domains of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteurs de transcription TCF › Protéine-1 de type facteur-7 de transcription › Structural basis of the bHLH domains of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Structural basis of the bHLH domains of...