Human CD38 regulates B cell antigen receptor dynamic organization in normal and malignant B cells.


Journal

The Journal of experimental medicine
ISSN: 1540-9538
Titre abrégé: J Exp Med
Pays: United States
ID NLM: 2985109R

Informations de publication

Date de publication:
05 09 2022
Historique:
received: 03 02 2022
revised: 11 05 2022
accepted: 13 06 2022
entrez: 12 7 2022
pubmed: 13 7 2022
medline: 15 7 2022
Statut: ppublish

Résumé

CD38 is a multifunctional protein expressed on the surface of B cells in healthy individuals but also in B cell malignancies. Previous studies have suggested a connection between CD38 and components of the IgM class B cell antigen receptor (IgM-BCR) and its coreceptor complex. Here, we provide evidence that CD38 is closely associated with CD19 in resting B cells and with the IgM-BCR upon engagement. We show that targeting CD38 with an antibody, or removing this molecule with CRISPR/Cas9, inhibits the association of CD19 with the IgM-BCR, impairing BCR signaling in normal and malignant B cells. Together, our data suggest that CD38 is a new member of the BCR coreceptor complex, where it exerts a modulatory effect on B cell activation upon antigen recognition by regulating CD19. Our study also reveals a new mechanism where α-CD38 antibodies could be a valuable option in therapeutic approaches to B cell malignancies driven by aberrant BCR signaling.

Identifiants

pubmed: 35819358
pii: 213348
doi: 10.1084/jem.20220201
pmc: PMC9280193
pii:
doi:

Substances chimiques

Adaptor Proteins, Signal Transducing 0
Antigens, CD19 0
Immunoglobulin M 0
Membrane Glycoproteins 0
Receptors, Antigen, B-Cell 0
CD38 protein, human EC 3.2.2.5
ADP-ribosyl Cyclase 1 EC 3.2.2.6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Swedish Research Council
ID : 2018-03128
Organisme : Swedish Cancer Foundation
ID : 19 0464
Organisme : Swedish Childhood Cancer Foundation
ID : PR2018-0170
Organisme : King Gustav V's 80-year Foundation
ID : SGI-2018-0510
Organisme : Elisabeth Bollan Lindén-stipendiet
Organisme : Assar Gabrielsson's Foundation
ID : FB19-66
Organisme : Wenner-Gren Foundation
Organisme : Reumatikerförbundet
Organisme : ALF
Organisme : Kungl. Vetenskaps- och Vitterhets-Samhället i Göteborg
Organisme : Adlerbertska Stiftelserna
Organisme : Stiftelsen Apotekare Hedbergs Fond för Medicinsk Forskning
Organisme : Stiftelsen Samariten
Organisme : German Research Foundation
ID : TRR130
Organisme : Roche Innovation Center Zurich
Organisme : Amlövs Stiftelser
Organisme : Lundgrens Stiftelse
Organisme : Göteborgsregionens Stiftelse för Reumatologisk Forskning
Organisme : Ingabritt och Arne Lundbergs Forskningsstiftelse

Informations de copyright

© 2022 Camponeschi et al.

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Auteurs

Alessandro Camponeschi (A)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Kathrin Kläsener (K)

Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany.
Signalling Research Centres Biological Signalling Studies and Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany.

Timothy Sundell (T)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Christina Lundqvist (C)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Paul T Manna (PT)

Department of Physiology, Institute of Neuroscience and Physiology, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Negar Ayoubzadeh (N)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Martina Sundqvist (M)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Katrin Thorarinsdottir (K)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Mariele Gatto (M)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Unit of Rheumatology, Department of Medicine, University of Padova, Padua, Italy.

Marcella Visentini (M)

Department of Translational and Precision Medicine, Sapienza University of Rome, Rome, Italy.

Karin Önnheim (K)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Alaitz Aranburu (A)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Huamei Forsman (H)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Olov Ekwall (O)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Pediatrics, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Linda Fogelstrand (L)

Department of Laboratory Medicine, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden.

Inger Gjertsson (I)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

Michael Reth (M)

Biology III, Faculty of Biology, University of Freiburg, Freiburg, Germany.
Signalling Research Centres Biological Signalling Studies and Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany.

Inga-Lill Mårtensson (IL)

Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.

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Classifications MeSH