Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process.
alpha-l-iduronidase
eGFP
hairy root
plant molecular farming
recombinant protein
secretion
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
23
05
2022
received:
28
02
2022
accepted:
07
06
2022
pubmed:
14
7
2022
medline:
9
9
2022
entrez:
13
7
2022
Statut:
ppublish
Résumé
Hairy root systems have proven to be a viable alternative for recombinant protein production. For recalcitrant proteins, maximizing the productivity of hairy root cultures is essential. The aim of this study was to optimize a Brassica rapa rapa hairy root process for secretion of alpha- l-iduronidase (IDUA), a biologic of medical value. The process was first optimized with hairy roots expressing eGFP. For the biomass optimization, the highest biomass yields were achieved in modified Gamborg B5 culture medium. For the secretion induction, the optimized secretion media was obtained with additives (1.5 g/l PVP + 1 mg/l 2,4- d + 20.5 g/l KNO
Identifiants
pubmed: 35822204
doi: 10.1002/bit.28178
pmc: PMC9543041
doi:
Substances chimiques
Biological Products
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2831-2841Informations de copyright
© 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.
Références
Plant Biotechnol J. 2019 Feb;17(2):505-516
pubmed: 30058762
Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7387-9
pubmed: 11607490
J Exp Bot. 2005 Oct;56(420):2611-8
pubmed: 16105856
Plant Biotechnol J. 2016 Feb;14(2):615-24
pubmed: 26038982
Annu Rev Entomol. 2002;47:845-81
pubmed: 11729093
FEBS Lett. 1998 Oct 23;437(3):319-23
pubmed: 9824316
FASEB J. 2009 Oct;23(10):3581-9
pubmed: 19470800
J Biotechnol. 2020 Nov 10;323:302-312
pubmed: 32682804
Mol Biotechnol. 2019 Feb;61(2):84-92
pubmed: 30484145
Nat Commun. 2012;3:1062
pubmed: 22990858
Biotechnol Bioeng. 2014 Feb;111(2):336-46
pubmed: 24030771
Biotechnol Bioeng. 2022 Oct;119(10):2831-2841
pubmed: 35822204
Front Plant Sci. 2018 Jan 26;9:45
pubmed: 29434617
Biotechnol Bioeng. 1997 Jun 5;54(5):401-15
pubmed: 18634133
Biotechnol Bioeng. 2010 Oct 1;107(2):278-89
pubmed: 20506104
Protein Expr Purif. 1996 Mar;7(2):220-8
pubmed: 8812866
Front Plant Sci. 2020 Mar 03;11:33
pubmed: 32194578
Plant Physiol. 2004 Sep;136(1):2483-99
pubmed: 15375205
Biotechnol Bioeng. 1999 Mar;62(6):711-721
pubmed: 10099574
Biotechnol Lett. 2014 Jan;36(1):181-90
pubmed: 24078130
Eng Life Sci. 2019 Jul 25;19(12):880-895
pubmed: 32624980
Biotechnol J. 2012 Apr;7(4):537-45
pubmed: 22125283
Biotechnol Prog. 2001 Nov-Dec;17(6):979-92
pubmed: 11735430
Viruses. 2015 Jul 30;7(8):4254-81
pubmed: 26264020
Plant Mol Biol. 2003 May;52(1):233-41
pubmed: 12825702