Fundamental insights in early-stage inclusion body formation.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
05 2023
05 2023
Historique:
revised:
29
06
2022
received:
10
06
2022
accepted:
30
06
2022
medline:
27
4
2023
pubmed:
14
7
2022
entrez:
13
7
2022
Statut:
ppublish
Résumé
Early-stage inclusion body formation is still mysterious. Literature is ambiguous about the existence of rod-shaped protein aggregates, a potential sponge-like inclusion body scaffold as well as the number of inclusion bodies per Escherichia coli cell. In this study, we verified the existence of rod-shaped inclusion bodies, confirmed their porous morphology, the presence of multiple protein aggregates per cell and modelled inclusion body formation as function of the number of generations.
Identifiants
pubmed: 35830603
doi: 10.1111/1751-7915.14117
pmc: PMC10128139
doi:
Substances chimiques
Protein Aggregates
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
893-900Informations de copyright
© 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
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