Phagocytosis of Plasmodium falciparum ring-stage parasites predicts protection against malaria.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 07 2022
14 07 2022
Historique:
received:
08
02
2021
accepted:
27
06
2022
entrez:
14
7
2022
pubmed:
15
7
2022
medline:
19
7
2022
Statut:
epublish
Résumé
Ring-infected erythrocytes are the predominant asexual stage in the peripheral circulation but are rarely investigated in the context of acquired immunity against Plasmodium falciparum malaria. Here we compare antibody-dependent phagocytosis of ring-infected parasite cultures in samples from a controlled human malaria infection (CHMI) study (NCT02739763). Protected volunteers did not develop clinical symptoms, maintained parasitaemia below a predefined threshold of 500 parasites/μl and were not treated until the end of the study. Antibody-dependent phagocytosis of both ring-infected and uninfected erythrocytes from parasite cultures was strongly correlated with protection. A surface proteomic analysis revealed the presence of merozoite proteins including erythrocyte binding antigen-175 and -140 on ring-infected and uninfected erythrocytes, providing an additional antibody-mediated protective mechanism for their activity beyond invasion-inhibition. Competition phagocytosis assays support the hypothesis that merozoite antigens are the key mediators of this functional activity. Targeting ring-stage parasites may contribute to the control of parasitaemia and prevention of clinical malaria.
Identifiants
pubmed: 35835738
doi: 10.1038/s41467-022-31640-6
pii: 10.1038/s41467-022-31640-6
pmc: PMC9281573
doi:
Substances chimiques
Antibodies, Protozoan
0
Antigens, Protozoan
0
Banques de données
ClinicalTrials.gov
['NCT02739763']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4098Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : EPA
ID : EP-C-15-003
Pays : United States
Organisme : Wellcome Trust
ID : 107499
Pays : United Kingdom
Organisme : Department of Health
ID : 16/136/33
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 107769/Z/10/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 107754/Z/15/Z
Pays : United Kingdom
Investigateurs
Abdirahman I Abdi
(AI)
Yonas Abebe
(Y)
Philip Bejon
(P)
Peter F Billingsley
(PF)
Peter C Bull
(PC)
Zaydah de Laurent
(Z)
Stephen L Hoffman
(SL)
Eric R James
(ER)
Silvia Kariuki
(S)
Rinter Kimathi
(R)
Sam Kinyanjui
(S)
Cheryl Kivisi
(C)
Johnstone Makale
(J)
Kevin Marsh
(K)
Khadija Said Mohammed
(KS)
Moses Mosobo
(M)
Janet Musembi
(J)
Jennifer Musyoki
(J)
Michelle Muthui
(M)
Jedidah Mwacharo
(J)
Francis Ndungu
(F)
Joyce M Ngoi
(JM)
Omar Ngoto
(O)
Bernhards Ogutu
(B)
Fredrick Olewe
(F)
Donwilliams Omuoyo
(D)
John Ong'echa
(J)
Edward Otieno
(E)
Jimmy Shangala
(J)
Betty Kim Lee Sim
(BKL)
Thomas L Richie
(TL)
Juliana Wambua
(J)
Thomas N Williams
(TN)
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022. The Author(s).
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