Mitochondrial prohibitin complex regulates fungal virulence via ATG24-assisted mitophagy.


Journal

Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179

Informations de publication

Date de publication:
14 07 2022
Historique:
received: 18 11 2021
accepted: 30 06 2022
entrez: 14 7 2022
pubmed: 15 7 2022
medline: 19 7 2022
Statut: epublish

Résumé

Prohibitins are highly conserved eukaryotic proteins in mitochondria that function in various cellular processes. The roles of prohibitins in fungal virulence and their regulatory mechanisms are still unknown. Here, we identified the prohibitins ChPhb1 and ChPhb2 in a plant pathogenic fungus Colletotrichum higginsianum and investigated their roles in the virulence of this anthracnose fungus attacking crucifers. We demonstrate that ChPhb1 and ChPhb2 are required for the proper functioning of mitochondria, mitophagy and virulence. ChPhb1 and ChPhb2 interact with the autophagy-related protein ChATG24 in mitochondria, and ChATG24 shares similar functions with these proteins in mitophagy and virulence, suggesting that ChATG24 is involved in prohibitin-dependent mitophagy. ChPhb1 and ChPhb2 modulate the translocation of ChATG24 into mitochondria during mitophagy. The role of ChATG24 in mitophagy is further confirmed to be conserved in plant pathogenic fungi. Our study presents that prohibitins regulate fungal virulence by mediating ATG24-assisted mitophagy.

Identifiants

pubmed: 35835849
doi: 10.1038/s42003-022-03666-5
pii: 10.1038/s42003-022-03666-5
pmc: PMC9283515
doi:

Substances chimiques

Prohibitins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

698

Informations de copyright

© 2022. The Author(s).

Références

EMBO J. 1994 Aug 15;13(16):3782-92
pubmed: 8070406
New Phytol. 2018 Jul;219(2):654-671
pubmed: 29676464
Biotech Histochem. 2010 Apr;85(2):99-105
pubmed: 19669979
Int J Mol Sci. 2018 Jul 23;19(7):
pubmed: 30041456
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
Environ Microbiol. 2018 Sep;20(9):3309-3324
pubmed: 30058280
Antimicrob Agents Chemother. 2018 Oct 24;62(11):
pubmed: 30181366
Cell Res. 2014 Jul;24(7):787-95
pubmed: 24903109
J Biol Chem. 2003 Aug 22;278(34):32091-9
pubmed: 12794069
Dev Cell. 2009 Jul;17(1):98-109
pubmed: 19619495
Trends Endocrinol Metab. 2009 Oct;20(8):394-401
pubmed: 19733482
Mol Biol Cell. 2005 Jan;16(1):248-59
pubmed: 15525670
Virulence. 2019 Dec;10(1):481-489
pubmed: 30475080
Curr Biol. 1997 Aug 1;7(8):607-10
pubmed: 9259555
Plant Signal Behav. 2019;14(11):1656036
pubmed: 31429630
Genes Dev. 2008 Feb 15;22(4):476-88
pubmed: 18281461
Mol Plant Microbe Interact. 2004 Mar;17(3):272-82
pubmed: 15000394
iScience. 2019 Sep 27;19:1065-1078
pubmed: 31522117
Dev Cell. 2013 Jul 15;26(1):9-18
pubmed: 23810512
Mol Cell Biol. 1998 Jul;18(7):4043-52
pubmed: 9632789
Front Microbiol. 2020 May 08;11:763
pubmed: 32457707
Plant J. 2006 May;46(4):658-67
pubmed: 16640602
Cell. 2017 Jan 12;168(1-2):224-238.e10
pubmed: 28017329
Cell Death Differ. 2005 Nov;12 Suppl 2:1542-52
pubmed: 16247502
Mol Plant Pathol. 2019 Aug;20(8):1147-1162
pubmed: 31218796
J Appl Microbiol. 2020 Aug;129(2):411-421
pubmed: 32086853
FEBS Lett. 1995 Jan 30;358(3):273-7
pubmed: 7843414
Biochemistry. 1986 Jul 29;25(15):4438-43
pubmed: 3092856
Autophagy. 2013 Nov 1;9(11):1818-27
pubmed: 23958498
Int J Mol Sci. 2018 Feb 13;19(2):
pubmed: 29438347
Autophagy. 2007 Mar-Apr;3(2):106-16
pubmed: 17204848
Protein Sci. 2002 Oct;11(10):2471-8
pubmed: 12237468
J Cell Biol. 2009 Feb 23;184(4):583-96
pubmed: 19221197
Environ Microbiol. 2022 Mar;24(3):1093-1116
pubmed: 34472183
Nature. 2015 May 28;521(7553):525-8
pubmed: 25896323
J Cell Sci. 2009 Nov 1;122(Pt 21):3823-30
pubmed: 19889967
Eukaryot Cell. 2012 Sep;11(9):1154-66
pubmed: 22843561
Autophagy. 2013 Nov 1;9(11):1900-1
pubmed: 24025250
Cell Rep. 2018 Apr 17;23(3):756-767
pubmed: 29669282
Cells. 2018 Nov 29;7(12):
pubmed: 30501123
Front Cell Neurosci. 2016 Mar 30;10:76
pubmed: 27065339
Cell. 2012 Mar 16;148(6):1145-59
pubmed: 22424226
Trends Biochem Sci. 1999 Nov;24(11):425-7
pubmed: 10542406
J Biol Chem. 2008 Jan 25;283(4):1786-98
pubmed: 18039652
Mol Plant Microbe Interact. 2004 Jul;17(7):749-62
pubmed: 15242169
Nat Cell Biol. 2007 Oct;9(10):1102-9
pubmed: 17909521
Trends Cell Biol. 2007 Aug;17(8):394-402
pubmed: 17766116
Front Plant Sci. 2015 Jun 03;6:414
pubmed: 26089832
Nat Genet. 2012 Sep;44(9):1060-5
pubmed: 22885923
Dev Cell. 2009 Jul;17(1):87-97
pubmed: 19619494

Auteurs

Yaqin Yan (Y)

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.
Institute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Jintian Tang (J)

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, 310018, China.

Qinfeng Yuan (Q)

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.

Caiyun Liu (C)

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.

Xiaolin Chen (X)

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.

Hao Liu (H)

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.

Junbin Huang (J)

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China.

Chonglai Bao (C)

Institute of Vegetables Research, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.

Tom Hsiang (T)

School of Environmental Sciences, University of Guelph, Guelph, ON, N1G 2W1, Canada.

Lu Zheng (L)

State Key Laboratory of Agricultural Microbiology/Hubei Key Laboratory of Plant Pathology, Huazhong Agricultural University, Wuhan, 430070, China. luzheng@mail.hzau.edu.cn.

Articles similaires

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis.

Spencer D Shelton, Sara House, Luiza Martins Nascentes Melo et al.
1.00
DNA, Mitochondrial Humans Melanoma Mutation Neoplasm Metastasis

A dual role for PSIP1/LEDGF in T cell acute lymphoblastic leukemia.

Lisa Demoen, Filip Matthijssens, Lindy Reunes et al.
1.00
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma Animals Mice Humans Cell Line, Tumor
Adenosine Triphosphate Adenosine Diphosphate Mitochondrial ADP, ATP Translocases Binding Sites Mitochondria
Genome, Bacterial Virulence Phylogeny Genomics Plant Diseases

Classifications MeSH