E. coli Expression and Purification of Microbial and Viral Rhodopsins.

Escherichia coli / genetics Rhodopsin / chemistry Rhodopsins, Microbial / chemistry
Escherichia coli Membrane protein Production Recombinant Rhodopsin

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 20 7 2022
pubmed: 21 7 2022
medline: 23 7 2022
Statut: ppublish

Résumé

Microbial rhodopsins have become an indispensable tool for neurobiology. Of thousands of identified microbial rhodopsins, a minute fraction has been studied so far and they have shown remarkable functional diversity suggesting more great promises that this large family holds. Effective production of recombinant microbial and viral rhodopsins is a prerequisite for the success of functional and structural studies of these proteins. Escherichia coli (E. coli) are suitable for high yield expression of many of microbial and viral rhodopsins and they facilitate rapid exploration of this large protein family.

Identifiants

DOI: 10.1007/978-1-0716-2329-9_5 PMID: 35857225
pubmed: 35857225
doi: 10.1007/978-1-0716-2329-9_5
doi:

Substances chimiques

Rhodopsins, Microbial 0
Rhodopsin 9009-81-8

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

109-124

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Taras Balandin (T)

Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH, Jülich, Germany. t.balandin@fz-juelich.de.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich GmbH, Jülich, Germany. t.balandin@fz-juelich.de.

Dmytro Volkov (D)

Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH, Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich GmbH, Jülich, Germany.

Alexey Alekseev (A)

Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia.

Kirill Kovalev (K)

Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH, Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich GmbH, Jülich, Germany.
Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Russia.
Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France.
Institute of Crystallography, University of Aachen (RWTH), Aachen, Germany.

Dmitry Bratanov (D)

Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich GmbH, Jülich, Germany.
JuStruct: Jülich Center for Structural Biology, Forschungszentrum Jülich GmbH, Jülich, Germany.

Valentin Gordeliy (V)

Institut de Biologie Structurale (IBS), Université Grenoble Alpes, CEA, CNRS, Grenoble, France.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › E. coli Expression and Purification of...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Pigments rétiniens › Opsines › Opsines des bâtonnets › Rhodopsine › E. coli Expression and Purification of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Photorécepteurs microbiens › Rhodopsines microbiennes › E. coli Expression and Purification of...