CRISPR interference interrogation of COPD GWAS genes reveals the functional significance of desmoplakin in iPSC-derived alveolar epithelial cells.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
15 07 2022
15 07 2022
Historique:
entrez:
20
7
2022
pubmed:
21
7
2022
medline:
23
7
2022
Statut:
ppublish
Résumé
Genome-wide association studies (GWAS) have identified dozens of loci associated with chronic obstructive pulmonary disease (COPD) susceptibility; however, the function of associated genes in the cell type(s) affected in disease remains poorly understood, partly due to a lack of cell models that recapitulate human alveolar biology. Here, we apply CRISPR interference to interrogate the function of nine genes implicated in COPD by GWAS in induced pluripotent stem cell-derived type 2 alveolar epithelial cells (iAT2s). We find that multiple genes implicated by GWAS affect iAT2 function, including differentiation potential, maturation, and/or proliferation. Detailed characterization of the GWAS gene
Identifiants
pubmed: 35857525
doi: 10.1126/sciadv.abo6566
pmc: PMC9278866
doi:
Substances chimiques
Desmoplakins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
eabo6566Subventions
Organisme : NHLBI NIH HHS
ID : F30 HL147426
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL137927
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL148667
Pays : United States
Références
Nature. 2020 Nov;587(7835):619-625
pubmed: 33208946
Proc Natl Acad Sci U S A. 1985 Jan;82(1):119-23
pubmed: 3871521
Nat Commun. 2022 Jan 25;13(1):494
pubmed: 35078977
Nat Protoc. 2007;2(2):329-33
pubmed: 17406593
Clin Sci (Lond). 2014 Feb;126(4):253-65
pubmed: 24144354
Cell Res. 2002 Mar;12(1):9-18
pubmed: 11942415
J Cell Biol. 2018 Sep 3;217(9):3219-3235
pubmed: 29959233
J Invest Dermatol. 2019 Jun;139(6):1227-1236
pubmed: 30579854
Nat Methods. 2018 Aug;15(8):611-616
pubmed: 30013045
J Cell Biol. 2007 Jan 15;176(2):147-54
pubmed: 17227889
Nat Genet. 2019 Mar;51(3):481-493
pubmed: 30804560
Thorax. 1991 Dec;46(12):863-70
pubmed: 1792631
Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10
pubmed: 28965766
Sci Rep. 2018 Aug 8;8(1):11881
pubmed: 30089872
Nature. 2015 Jan 29;517(7536):583-8
pubmed: 25494202
Cell Rep. 2021 Aug 31;36(9):109636
pubmed: 34469722
Cell Stem Cell. 2016 Apr 7;18(4):541-53
pubmed: 26971820
F1000Res. 2019 Dec 30;8:
pubmed: 31942240
Eur Respir J. 2005 Feb;25(2):250-8
pubmed: 15684288
Nat Cell Biol. 2001 Dec;3(12):1076-85
pubmed: 11781569
Cell Stem Cell. 2020 Dec 3;27(6):962-973.e7
pubmed: 32979316
Am J Respir Crit Care Med. 2016 May 15;193(10):1151-60
pubmed: 26669357
Dis Model Mech. 2010 Sep-Oct;3(9-10):545-56
pubmed: 20699479
Mol Biol Cell. 2012 Mar;23(5):792-9
pubmed: 22238362
J Med Chem. 2011 May 12;54(9):3109-52
pubmed: 21504156
Mol Biol Cell. 2020 May 15;31(11):1140-1153
pubmed: 32238101
Nucleic Acids Res. 2014 Oct 29;42(19):e147
pubmed: 25122746
Nature. 2014 Mar 13;507(7491):190-4
pubmed: 24499815
Diabetes Metab Rev. 1998 Dec;14(4):263-83
pubmed: 10095997
Respir Res. 2016 Jul 29;17(1):95
pubmed: 27473260
Sci Adv. 2020 Jul 08;6(28):eaba1972
pubmed: 32832598
Cell Stem Cell. 2017 Jun 1;20(6):844-857.e6
pubmed: 28366587
Exp Cell Res. 2007 Jul 1;313(11):2336-44
pubmed: 17475244
J Vis Exp. 2022 Apr 15;(182):
pubmed: 35499347
Nature. 2022 Apr;604(7904):120-126
pubmed: 35355013
JCI Insight. 2022 Mar 22;7(6):
pubmed: 35315362
Proc Am Thorac Soc. 2012 Jul;9(3):111-6
pubmed: 22802283
Nature. 2018 Mar 8;555(7695):251-255
pubmed: 29489752
Genome Res. 2015 Dec;25(12):1860-72
pubmed: 26430063
Thorax. 2015 Sep;70(9):822-9
pubmed: 26048404
Nat Protoc. 2019 Dec;14(12):3303-3332
pubmed: 31732721
Am J Pathol. 2013 Jul;183(1):131-43
pubmed: 23665346
Nat Commun. 2018 Jan 2;9(1):20
pubmed: 29295995
N Engl J Med. 2015 Jul 9;373(2):111-22
pubmed: 26154786
Cell Mol Life Sci. 2011 Mar;68(5):877-92
pubmed: 20820852
Nat Genet. 2017 Mar;49(3):426-432
pubmed: 28166215
JAMA Intern Med. 2015 Sep;175(9):1539-49
pubmed: 26098755
Nat Genet. 2017 Mar;49(3):416-425
pubmed: 28166213
Carcinogenesis. 2012 Oct;33(10):1863-70
pubmed: 22791817
Nat Commun. 2020 Jan 31;11(1):635
pubmed: 32005814
Am J Respir Crit Care Med. 2020 Nov 1;202(9):1225-1236
pubmed: 32551799
Am J Respir Crit Care Med. 2016 Jul 15;194(2):185-97
pubmed: 26862784
Cancer Cell. 2012 Oct 16;22(4):547-60
pubmed: 23079663
Cell Stem Cell. 2020 Apr 2;26(4):593-608.e8
pubmed: 32004478
J Biol Chem. 1994 Nov 25;269(47):29351-4
pubmed: 7525582
Nat Commun. 2020 Dec 8;11(1):6283
pubmed: 33293539
Am J Respir Crit Care Med. 2013 Oct 15;188(8):941-7
pubmed: 23972146
Nat Genet. 2011 Sep 25;43(11):1082-90
pubmed: 21946350
J Clin Invest. 2013 Jul;123(7):3025-36
pubmed: 23921127
Nat Genet. 2019 Mar;51(3):494-505
pubmed: 30804561
J Clin Invest. 2017 Jun 1;127(6):2277-2294
pubmed: 28463226
Cell Stem Cell. 2014 Aug 7;15(2):215-226
pubmed: 24931489
Science. 2018 Mar 9;359(6380):1118-1123
pubmed: 29420258
Genome Biol. 2020 Feb 12;21(1):36
pubmed: 32051003