Selective and high-yield production of ethyl α-d-glucopyranoside by the α-glucosyl transfer enzyme of Xanthomonas campestris WU-9701 and glucose isomerase.

Aldose-Ketose Isomerases Ethanol Glucose Maltose Xanthomonas campestris
Alkyl α-glucoside Glucose isomerase Xanthomonas campestris α-Glucosidase α-Glucosyl transfer enzyme

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
Sep 2022
Historique:
received: 16 02 2022
revised: 21 06 2022
accepted: 22 06 2022
pubmed: 22 7 2022
medline: 16 9 2022
entrez: 21 7 2022
Statut: ppublish

Résumé

Ethyl α-d-glucopyranoside (α-EG) is detected in sake (Japanese rice wine), that has moisturizing and skin conditioning effects. The production of α-EG by fermentation or enzymatic synthesis to date generates unwanted by-products such as maltooligosaccharides and/or organic acids. In this study, we employed a reaction involving selective α-glucosylation of ethanol by the α-glucosyl transfer enzyme (XgtA) of Xanthomonas campestris WU-9701. Under standard conditions, when 0.80 M ethanol and 1.2 M maltose were used as substrates with XgtA (2.5 units) and incubated in 30 mM HEPES-NaOH buffer (pH 8.0) at 45°C, only one form of ethyl glucopyranoside was selectively obtained as a product. The isolated product was identified as ethyl α-d-glucopyranoside by

Identifiants

DOI: 10.1016/j.jbiosc.2022.06.012 PMID: 35864059
pubmed: 35864059
pii: S1389-1723(22)00165-7
doi: 10.1016/j.jbiosc.2022.06.012
pii:
doi:

Substances chimiques

Ethanol 3K9958V90M
Maltose 69-79-4
Aldose-Ketose Isomerases EC 5.3.1.-
xylose isomerase EC 5.3.1.5
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

220-225

Informations de copyright

Copyright © 2022 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Kohtaro Kirimura (K)

Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan; Waseda Research Institute for Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan. Electronic address: kkohtaro@waseda.jp.

Wei Cao (W)

Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.

Yutaka Onda (Y)

Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.

Isato Yoshioka (I)

Waseda Research Institute for Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.

Yoshitaka Ishii (Y)

Waseda Research Institute for Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Isomerases › Intramolecular oxidoreductases › Aldose-ketose isomerases › Selective and high-yield production of ethyl...
questionsmedicales.fr › Composés chimiques organiques › Alcools › Éthanol › Selective and high-yield production of ethyl...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Selective and high-yield production of ethyl...
questionsmedicales.fr › Glucides › Sucres › Diholoside › Maltose › Selective and high-yield production of ethyl...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Xanthomonadaceae › Xanthomonas › Xanthomonas campestris › Selective and high-yield production of ethyl...