Application of Green-enhanced Nano-lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH.
apoplast
bioluminescence
pH homoeostasis
pH indicator
root imaging
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
11
07
2022
received:
14
06
2022
accepted:
18
07
2022
pubmed:
22
7
2022
medline:
14
9
2022
entrez:
21
7
2022
Statut:
ppublish
Résumé
Plant root absorbs water and nutrients from the soil, and the root apoplastic fluid (AF) is an important intermediate between cells and the surrounding environment. The acid growth theory suggests that an acidic AF is needed for cell wall expansion during root growth. However, technical limitations have precluded the quantification of root apoplastic fluid pH (AF-pH). Here, we used Green-enhanced Nano-lantern (GeNL), a chimeric protein of the luciferase NanoLuc (Nluc) and the green fluorescent protein mNeonGreen (mNG), as a ratiometric pH indicator based on the pH dependency of bioluminescence resonance energy transfer efficiency from Nluc to mNG. Luminescence spectrum of GeNL changed reciprocally from pH 4.5 to 7.5, with a pK
Identifiants
pubmed: 35864560
doi: 10.1111/pce.14404
pmc: PMC9542637
doi:
Substances chimiques
Luciferases
EC 1.13.12.-
nanoluc
EC 1.13.12.-
Types de publication
Case Reports
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3157-3170Informations de copyright
© 2022 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.
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