HPLC analysis of carotenoids from bacteria.

Bacteria Carotenoids Chromatography, High Pressure Liquid / methods Spectrum Analysis
Carotenoid optical properties Carotenoids structures High performance liquid chromatography Quantification Separation Standard carotenoids

Journal

Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271

Informations de publication

Date de publication:
2022
Historique:
entrez: 25 7 2022
pubmed: 26 7 2022
medline: 27 7 2022
Statut: ppublish

Résumé

Bacteria synthesize different carotenoid structures. A powerful method for the determination of carotenoid profiles of bacteria, including quantification, is high performance liquid chromatography (HPLC). This chapter explains the basics of this method and its application, and provides instruction on how to proceed with HPLC. A basic HPLC system for bacterial carotenoids is presented which allows tentative identification and quantification. The individual analysis steps including extraction, sample preparation and HPLC separation are described in detail. HPLC examples of structurally diverse carotenoids from different bacterial phyla are displayed demonstrating the applicability of this method. In addition, information on spectroscopic properties useful for identification is included. Finally, a procedure for carotenoid quantification from the HPLC chromatogram is described.

Identifiants

DOI: 10.1016/bs.mie.2021.10.006 PMID: 35871834
pubmed: 35871834
pii: S0076-6879(21)00400-6
doi: 10.1016/bs.mie.2021.10.006
pii:
doi:

Substances chimiques

Carotenoids 36-88-4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

139-153

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Auteurs

Gerhard Sandmann (G)

Institute of Molecular Biosciences, Goethe University, Frankfurt, Germany. Electronic address: sandmann@bio.uni-frankfurt.de.

Articles similaires

Planting density effect on poplar growth traits and soil nutrient availability, and response of microbial community, assembly and function.

Rongye Qiao, Zhen Song, Yinglong Chen et al.
1.00
Populus Soil Microbiology Soil Microbiota Fungi

The effect of medical face masks on inhalation risk of bacterial bioaerosols in hospital waste decontamination station.

Morvarid Boroumand Alipour, Mojtaba Davoudi, Hadi Farsiani et al.
1.00
Aerosols Humans Decontamination Air Microbiology Masks

Comparative genomic analysis and characterization of novel high-quality draft genomes from the coal metagenome.

Arunmozhi Bharathi Achudhan, Lilly M Saleena
1.00
Coal Metagenome Phylogeny Bacteria Genome, Bacterial

Polymer semiconductor films and bacteria hybrid artificial bio-leaves.

Na Wen, Qianqing Jiang, Dianyi Liu
1.00
Semiconductors Photosynthesis Polymers Carbon Dioxide Bacteria

Classifications MeSH

questionsmedicales.fr › Bactéries › HPLC analysis of carotenoids from bacteria.
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Caroténoïdes › HPLC analysis of carotenoids from bacteria.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › HPLC analysis of carotenoids from bacteria.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › HPLC analysis of carotenoids from bacteria.