Stress-Tolerant, Recyclable, and Renewable Biocatalyst Platform Enabled by Engineered Bacterial Spores.

Bacillus subtilis / metabolism Bacterial Proteins / metabolism Enzymes, Immobilized / metabolism Recombinant Fusion Proteins / metabolism Recombinant Proteins / genetics Spores, Bacterial / genetics
bacterial spores biocatalysis enzyme immobilization protein engineering sustainable chemistry synthetic biology

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
19 08 2022
Historique:
pubmed: 26 7 2022
medline: 23 8 2022
entrez: 25 7 2022
Statut: ppublish

Résumé

Here, we describe a stress-tolerant, recyclable, and renewable biocatalyst platform based on T7 RNA polymerase-enabled high-density protein display on bacterial spores (TIED). TIED uses high-level T7 RNA polymerase-driven expression of recombinant proteins specifically in sporulating cells to allow spontaneous assembly of recombinant fusion proteins on the

Identifiants

DOI: 10.1021/acssynbio.2c00256 PMID: 35878063
pubmed: 35878063
doi: 10.1021/acssynbio.2c00256
doi:

Substances chimiques

Bacterial Proteins 0
Enzymes, Immobilized 0
Recombinant Fusion Proteins 0
Recombinant Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2857-2868

Auteurs

Yue Hui (Y)

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, United States.
Department of Chemistry, University of California Irvine, Irvine, California 92697, United States.

Ziyu Cui (Z)

Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California 92697, United States.

Seunghyun Sim (S)

Department of Chemistry, University of California Irvine, Irvine, California 92697, United States.
Department of Chemical and Biomolecular Engineering, University of California Irvine, Irvine, California 92697, United States.
Department of Biomedical Engineering, University of California Irvine, Irvine, California 92697, United States.
ORCID: 0000-0002-4232-5917

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus subtilis › Stress-Tolerant, Recyclable, and Renewable...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Stress-Tolerant, Recyclable, and Renewable...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines immobilisées › Enzymes immobilisées › Stress-Tolerant, Recyclable, and Renewable...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Protéines de fusion recombinantes › Stress-Tolerant, Recyclable, and Renewable...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Stress-Tolerant, Recyclable, and Renewable...
questionsmedicales.fr › Stades d'organismes › Spores › Spores bactériens › Stress-Tolerant, Recyclable, and Renewable...