Solid fraction determines stiffness and viscosity in decellularized pancreatic tissues.

Extracellular Matrix / chemistry Pancreas / diagnostic imaging Pancreatic Hormones / analysis Viscosity
Decellularization Decellularized pancreas MR elastography Solid fraction Viscoelastic models

Journal

Biomaterials advances
ISSN: 2772-9508
Titre abrégé: Biomater Adv
Pays: Netherlands
ID NLM: 9918383886206676

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 15 04 2022
revised: 05 06 2022
accepted: 20 06 2022
pubmed: 27 7 2022
medline: 11 8 2022
entrez: 26 7 2022
Statut: ppublish

Résumé

The role of extracellular matrix (ECM) composition and turnover in mechano-signaling and the metamorphic fate of cells seeded into decellularized tissue can be elucidated by recent developments in non-invasive imaging and biotechnological analysis methods. Because these methods allow accurate quantification of the composition and structural integrity of the ECM, they can be critical in establishing standardized decellularization protocols. This study proposes quantification of the solid fraction, the single-component fraction and the viscoelasticity of decellularized pancreatic tissues using compact multifrequency magnetic resonance elastography (MRE) to assess the efficiency and quality of decellularization protocols. MRE of native and decellularized pancreatic tissues showed that viscoelasticity parameters depend according to a power law on the solid fraction of the decellularized matrix. The parameters can thus be used as highly sensitive markers of the mechanical integrity of soft tissues. Compact MRE allows consistent and noninvasive quantification of the viscoelastic properties of decellularized tissue. Such a method is urgently needed for the standardized monitoring of decellularization processes, evaluation of mechanical ECM properties, and quantification of the integrity of solid structural elements remaining in the decellularized tissue matrix.

Identifiants

DOI: 10.1016/j.bioadv.2022.212999 PMID: 35882147
pubmed: 35882147
pii: S2772-9508(22)00276-X
doi: 10.1016/j.bioadv.2022.212999
pii:
doi:

Substances chimiques

Pancreatic Hormones 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

212999

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Joachim Snellings (J)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Radiology, Charitéplatz 1, 10117 Berlin, Germany.

Eriselda Keshi (E)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany.

Peter Tang (P)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany.

Assal Daneshgar (A)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany.

Esther C Willma (EC)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany.

Luna Haderer (L)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany.

Oliver Klein (O)

Berlin Institute of Health at Charité - Universitätsmedizin Berlin, BIH Center for Regenerative Therapies, Charitéplatz 1, 10117 Berlin, Germany.

Felix Krenzien (F)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany; Berlin Institute of Health, Germany at Charité - Universitätsmedizin Berlin, BIH Acadamy, Clinician Scientist Program, Charitéplatz 1, 10117 Berlin, Germany.

Thomas Malinka (T)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany.

Patrick Asbach (P)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Radiology, Charitéplatz 1, 10117 Berlin, Germany.

Johann Pratschke (J)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany; Cluster of Excellence "Matters of Activity. Image Space Material" funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - E.XC 2025, Germany.

Igor M Sauer (IM)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany; Cluster of Excellence "Matters of Activity. Image Space Material" funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - E.XC 2025, Germany.

Jürgen Braun (J)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Institute for Medical Informatics, Charitéplatz 1, 10117 Berlin, Germany.

Ingolf Sack (I)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Radiology, Charitéplatz 1, 10117 Berlin, Germany; Cluster of Excellence "Matters of Activity. Image Space Material" funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - E.XC 2025, Germany. Electronic address: ingolf.sack@charite.de.

Karl Hillebrandt (K)

Charité - Universitätsmedizin Berlin, Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Surgery, Campus Charité Mitte|Campus Virchow-Klinikum, Charitéplatz 1, 10117 Berlin, Germany; Berlin Institute of Health, Germany at Charité - Universitätsmedizin Berlin, BIH Acadamy, Clinician Scientist Program, Charitéplatz 1, 10117 Berlin, Germany; Cluster of Excellence "Matters of Activity. Image Space Material" funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany's Excellence Strategy - E.XC 2025, Germany.

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Classifications MeSH

questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Solid fraction determines stiffness and...
questionsmedicales.fr › Système digestif › Pancréas › Solid fraction determines stiffness and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones pancréatiques › Solid fraction determines stiffness and...
questionsmedicales.fr › Phénomènes chimiques › Viscosité › Solid fraction determines stiffness and...