A quantification method of somatic mutations in normal tissues and their accumulation in pediatric patients with chemotherapy.
chemotherapy
duplex sequencing
next-generation sequencing
normal tissue
somatic mutation
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
02 08 2022
02 08 2022
Historique:
entrez:
27
7
2022
pubmed:
28
7
2022
medline:
30
7
2022
Statut:
ppublish
Résumé
Somatic mutations are accumulated in normal human tissues with aging and exposure to carcinogens. If we can accurately count any passenger mutations in any single DNA molecule, since their quantity is much larger than driver mutations, we can sensitively detect mutation accumulation in polyclonal normal tissues. Duplex sequencing, which tags both DNA strands in one DNA molecule, enables accurate count of such mutations, but requires a very large number of sequencing reads for each single sample of human-genome size. Here, we reduced the genome size to 1/90 using the
Identifiants
pubmed: 35895679
doi: 10.1073/pnas.2123241119
pmc: PMC9351471
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2123241119Références
Nature. 2013 Jul 11;499(7457):214-218
pubmed: 23770567
Nucleic Acids Res. 2019 Jan 8;47(D1):D941-D947
pubmed: 30371878
Genome Biol. 2016 Oct 3;17(1):205
pubmed: 27716309
Gene. 1986;42(1):119-23
pubmed: 3013727
Genet Anal Tech Appl. 1990 Dec;7(8):212-8
pubmed: 2151115
Nat Rev Genet. 2018 May;19(5):269-285
pubmed: 29576615
J Mol Biol. 1974 May 5;85(1):51-65
pubmed: 4365619
Leukemia. 2014 Dec;28(12):2276-82
pubmed: 25005245
Nat Rev Cancer. 2002 Feb;2(2):124-32
pubmed: 12635175
Nature. 2016 Oct 13;538(7624):260-264
pubmed: 27698416
Science. 2018 Nov 23;362(6417):911-917
pubmed: 30337457
J Natl Cancer Inst. 2001 Apr 18;93(8):618-29
pubmed: 11309438
Clin Cancer Res. 2004 Oct 15;10(20):6897-904
pubmed: 15501967
Science. 1993 Feb 12;259(5097):946-51
pubmed: 8438152
Proc Natl Acad Sci U S A. 2016 Aug 30;113(35):9846-51
pubmed: 27528664
Genome Res. 2011 Jul;21(7):1087-98
pubmed: 21628451
G3 (Bethesda). 2014 Oct 27;4(12):2483-92
pubmed: 25352541
Hematology Am Soc Hematol Educ Program. 2007;:453-9
pubmed: 18024664
Science. 2020 Oct 2;370(6512):82-89
pubmed: 33004515
Science. 2015 May 22;348(6237):880-6
pubmed: 25999502
Blood. 2002 Oct 1;100(7):2292-302
pubmed: 12239137
Cancer Lett. 2017 Sep 10;403:152-158
pubmed: 28642169
NPJ Precis Oncol. 2019 Mar 6;3:7
pubmed: 30854468
Nat Rev Cancer. 2017 Aug 24;17(9):513-527
pubmed: 28835720
Semin Oncol. 2013 Dec;40(6):666-75
pubmed: 24331189
Trends Biotechnol. 2018 Jul;36(7):729-740
pubmed: 29550161
Science. 2021 Sep 24;373(6562):1474-1479
pubmed: 34554797
Nature. 2020 Feb;578(7794):266-272
pubmed: 31996850
Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14508-13
pubmed: 22853953
Cell. 2019 May 2;177(4):821-836.e16
pubmed: 30982602
JAMA. 2011 Jun 8;305(22):2311-9
pubmed: 21642683
Nature. 2019 Jan;565(7739):312-317
pubmed: 30602793
PLoS One. 2018 Oct 2;13(10):e0204265
pubmed: 30278055
Arch Toxicol. 2019 Sep;93(9):2689-2701
pubmed: 31451845
Proc Natl Acad Sci U S A. 1989 Oct;86(20):7971-5
pubmed: 2530578
Nat Commun. 2018 Feb 23;9(1):800
pubmed: 29476074
Nature. 2001 Feb 15;409(6822):860-921
pubmed: 11237011
Nature. 2021 May;593(7859):405-410
pubmed: 33911282
Nature. 2013 Aug 22;500(7463):415-21
pubmed: 23945592
Nat Commun. 2021 Aug 10;12(1):4803
pubmed: 34376657
Nat Protoc. 2014 Nov;9(11):2586-606
pubmed: 25299156
Nat Methods. 2008 Mar;5(3):247-52
pubmed: 18297082
Genome Biol. 2019 Dec 18;20(1):285
pubmed: 31849330